Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou

YANG Ji; LONG Qi-Xin; WU Wen-Yan; WANG Xun-Zhang

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February 2000

YANG Ji, LONG Qi-Xin, WU Wen-Yan and WANG Xun-Zhang. Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou[J]. Virologica Sinica, 2000, 15(1).

Citation: YANG Ji, LONG Qi-Xin, WU Wen-Yan, WANG Xun-Zhang. Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou .VIROLOGICA SINICA, 2000, 15(1) : 45.

斜纹夜蛾核型多角体病毒p~(74)基因的克隆和序列分析

1.
中山大学昆虫所广州

摘要

用AcNPV p74基因 3′端的 1.4kb片段 ,通过Southernblotting将SpltNPV的 p74基因定位于XhoI(4 .9kb)、EcoRI(4 .4kb)、BamHI(3.0kb)片段上。进一步用ExoⅢ和构建亚克隆测序法 ,对长 2 545bp的 p74基因进行了序列分析 ,其中 1974bp的编码区编码 6 58个氨基酸 ,蛋白分子量约 75.87kD ,其编码蛋白与AcMNPV和CfMNPV p74蛋白的氨基酸序列同源性分别为 6 4 %和6 3%。
- 斜纹夜蛾核型多角体病毒（spltNPV） p（74）基因基因克隆序列测定

Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou

1.
Insect Institute,Sun Yat-san University

Abstract

The p74 gene of SpltNPV was located at EcoRI 4.4 kb,XhoI 4.9 kb and BamHI 3.0 kb fragments by Southern blotting with AcMNPV derived probe. After cloning of the three fragments, restriction enzyme analysis was used to construct their restriction map. A region of 2?545 bp including p74 gene of SpltNPV was sequenced. The open reading frame of SpltNPV p74 gene was decided to be 1?974 bp long, which codes 658 amino acids. Comparison of the homology with other baculovirus p74 genes shows that SpltNPV p74 is 64% and 63% identical to that of AcMNPV and CfMNPV at the amino acid level.
- SpltNPV p74 gene Gene cloning Sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on genetic and biological character of sporadic strains of acute HEV in Guangzhou

1. Insect Institute,Sun Yat-san University

Abstract: The p74 gene of SpltNPV was located at EcoRI 4.4 kb,XhoI 4.9 kb and BamHI 3.0 kb fragments by Southern blotting with AcMNPV derived probe. After cloning of the three fragments, restriction enzyme analysis was used to construct their restriction map. A region of 2?545 bp including p74 gene of SpltNPV was sequenced. The open reading frame of SpltNPV p74 gene was decided to be 1?974 bp long, which codes 658 amino acids. Comparison of the homology with other baculovirus p74 genes shows that SpltNPV p74 is 64% and 63% identical to that of AcMNPV and CfMNPV at the amino acid level.