Biological and M olecular Biological Characterization of W heat Yellow M osaic an d W heat Spindle Streak Mosaic Bymovirases

XIE Lian; YANG Shou-Jing; LIU Pan-Fang

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April 2000

XIE Lian, YANG Shou-Jing and LIU Pan-Fang. Biological and M olecular Biological Characterization of W heat Yellow M osaic an d W heat Spindle Streak Mosaic Bymovirases[J]. Virologica Sinica, 2000, 15(2): 97-105.

Citation: XIE Lian, YANG Shou-Jing, LIU Pan-Fang. Biological and M olecular Biological Characterization of W heat Yellow M osaic an d W heat Spindle Streak Mosaic Bymovirases .VIROLOGICA SINICA, 2000, 15(2) : 97-105.

汉滩病毒感染诱导热休克蛋白70表达

1.
浙江大学生物科学与技术系，杭州310027

摘要

为了解汉滩病毒感染后细胞的应激反应及HSP70的表达与病毒复制的关系,在汉滩病毒A9株感染VeroE6细胞后,用免疫组织化学及核酸分子原位杂交法,对细胞HSP70基因的表达进行了检测。结果表明,汉滩病毒感染细胞4h后即可诱导VeroE6细胞表达HSP70,表达可持续至感染后5d,且HSP70在细胞内的分布也有改变。提示汉滩病毒可直接诱导HSP70的高表达
- 热休克蛋白
- , 汉滩病毒
- , Vero-E6细胞

Biological and M olecular Biological Characterization of W heat Yellow M osaic an d W heat Spindle Streak Mosaic Bymovirases

1.
Department of Biological Science and Technology，ZJtejiang University,Hanzhou 310027,China

Abstract

In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results show that the expression of HSP70 can be induced in the cells 4 hours after Hantaan virus infection and lasted until the fifth days after infection. The distribution of HSP70 also varied. It suggests that the expression of HSP70 can be induced directly by Hantaan virus.
- wheat yellow raosa c yl[nOV
- , Wheat spindle streakbymovitrus
- , Epidemiology

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Biological and M olecular Biological Characterization of W heat Yellow M osaic an d W heat Spindle Streak Mosaic Bymovirases

1. Department of Biological Science and Technology，ZJtejiang University,Hanzhou 310027,China

Abstract: In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results show that the expression of HSP70 can be induced in the cells 4 hours after Hantaan virus infection and lasted until the fifth days after infection. The distribution of HSP70 also varied. It suggests that the expression of HSP70 can be induced directly by Hantaan virus.