Study on Human Cytomegalovirus(HCMV)Infection in the Blood Donors

LI Chun-Yang; LV Chun-Lan; YU Dong-Wei; ZHANG Li-Beng; SAI Wen; TUN Bing-Ren

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April 2000

LI Chun-Yang, LV Chun-Lan, YU Dong-Wei, ZHANG Li-Beng, SAI Wen and TUN Bing-Ren. Study on Human Cytomegalovirus(HCMV)Infection in the Blood Donors[J]. Virologica Sinica, 2000, 15(2): 111-115.

Citation: LI Chun-Yang, LV Chun-Lan, YU Dong-Wei, ZHANG Li-Beng, SAI Wen, TUN Bing-Ren. Study on Human Cytomegalovirus(HCMV)Infection in the Blood Donors .VIROLOGICA SINICA, 2000, 15(2) : 111-115.

献血员巨细胞病毒感染的研究

1.
内蒙古自治区卫生防疫站,呼和浩特 010020

摘要

用PCR法和DNA杂交法检测同一献血员的白细胞及血清中的HCMVDNA,并用ELISA法检测血清中的HCMVIgM、IgG(测四个滴度),连续两年共检测白细胞和血清样本各200人份。PCR法检测白细胞中的HCMVDNA阳性率分别为63%和70%,DNA杂交法检测的阳性率为42%和50%。PCR法检测血清中的HCMVDNA的阳性率为49%和53%,DNA杂交法检测的阳性率为33%和39%。HCMVIgM阳性率两年均为5%。HCMVIgG阳性率分别为54%和58%
- 献血员
- , 白细胞
- , 血清
- , 巨细胞病毒
- , 抗体

Study on Human Cytomegalovirus(HCMV)Infection in the Blood Donors

1.
Inner Mongolia Health and Anti-epidemic Station，Huhtaot 010020

Abstract

HCMVs in blood leukocytes and in serum from the same blood donor were examined by polymerase chain reaction (PCR) and DNA hybridization technique. In the mean time, HCMV IgM and HCMV IgG in the serum were examined by ELISA. Two hundred blood leukocyte samples and 200 serum samples were examined in a successive two years period. HCMV positive rates for the blood leukocytes by PCR in the different years were 63% and 70% while the positive rates by DNA hybridization were 42% and 50%, respectively. HCMV postive rates for the serum by PCR in the different years were 49% and 53% while the positive rates by DNA hybridization were 33% and 39%, respectively. A 5% HCMV IgM positive rate was found in the two years while 54% and 58% HCMV IgG positive rates were found in the two different years.
- Blood donor
- , Leukocyte
- , Serum
- , HCM V
- , Antibody

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on Human Cytomegalovirus(HCMV)Infection in the Blood Donors

1. Inner Mongolia Health and Anti-epidemic Station，Huhtaot 010020

Abstract: HCMVs in blood leukocytes and in serum from the same blood donor were examined by polymerase chain reaction (PCR) and DNA hybridization technique. In the mean time, HCMV IgM and HCMV IgG in the serum were examined by ELISA. Two hundred blood leukocyte samples and 200 serum samples were examined in a successive two years period. HCMV positive rates for the blood leukocytes by PCR in the different years were 63% and 70% while the positive rates by DNA hybridization were 42% and 50%, respectively. HCMV postive rates for the serum by PCR in the different years were 49% and 53% while the positive rates by DNA hybridization were 33% and 39%, respectively. A 5% HCMV IgM positive rate was found in the two years while 54% and 58% HCMV IgG positive rates were found in the two different years.