利用受精卵原核显微注射的方法,产生了含有HGV结构蛋白C、E1、E2及部分非结构蛋白NS2、NS3的转基因小鼠。得到10只founder小鼠,其中有3只founder小鼠与正常小鼠交配后得到了整合有外源基因的F1代阳性小鼠。RTPCR的结果显示,外源基因可在founder小鼠及F1代小鼠的血液有核细胞及肝细胞内转录;组织病理学检查显示,某些转基因小鼠的肝细胞出现了水样变、脂肪变性及轻微炎性反应等病理学改变,但与同一品系的正常小鼠相比,转基因小鼠血清转氨酶无明显升高。

The target fragment of HGV genes including C, E1, E2, NS2 and partial NS3 regions was microinjected into the pronuclei of mouse fertilized eggs according to the standard methods. Ten mice containing the HGV genes were identified by the screening of polymerase chain reaction with the primers in NS3 region. The integrated HGV genes were vertically transmitted to the offspring, and the F 1 heterozygous mice from three founders were detected positive. The results of RT PCR indicated that the HGV genes can be transcripted in blood monocytes and hepatocytes. The conventional pathological observation suggested that the transgenic mice had the cloudy swelling, steatosis, hydropic change and inflammatory cellular infiltration. However, serum ATL level showed no significant difference between transgenic and normal mice.