Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4

WEI Ta-Yun; LIN Han-Xin; TUN Jie-Jian; LIN Ai-Yang; XIE Lian-Hui

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April 2000

WEI Ta-Yun, LIN Han-Xin, TUN Jie-Jian, LIN Ai-Yang and XIE Lian-Hui. Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4[J]. Virologica Sinica, 2000, 15(2): 156-162.

Citation: WEI Ta-Yun, LIN Han-Xin, TUN Jie-Jian, LIN Ai-Yang, XIE Lian-Hui. Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4 .VIROLOGICA SINICA, 2000, 15(2) : 156-162.

水稻条纹病毒两个分离物RNA4基因间隔区的序列比较

1.
福建农业大学植物病毒研究所，福建省植物病毒学重点实验室福州350002

摘要

通过RTPCR扩增了我国水稻条纹病毒(RSV)山东济宁(JN)、云南宜良(YL)两个分离物RNA4基因间隔区(intergenicregion,IR)序列,并克隆于pGEMTeasy载体上。序列分析结果表明:JN、YL两分离物RNA4IR均由654个核苷酸组成,两者之间的同源率为92%。JN、YL两个分离物RNA4IR在AU碱基富集处可形成两个明显的发夹结构,其中一个序列比较保守,形成的发夹结构稳定;但另一个由于碱基变异导致所形成的发夹结构的稳定性在各个分离物中差异较大。这些结果说明了我国水稻条纹病毒存在明显的分子变异
- 水稻条纹病毒
- , RNA4基因间隔区
- , 序列比较

Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4

1.
Institute of Plant Virologyo of Fujian Agricuhurat University ,Key Laboratory of Plant Virology of Fujian Province，Fuzhou 350002,China

Abstract

The RNA4 intergenic regions of two isolates of rice stripe virus (RSV) in China (de signated as RSV JN and RSV YL) were amplified by RT PCR and cloned into pGEM T easy vector. Sequence analysis showed that they were all 654 bp in length, and shared only 92% identity at the nucleotide level. Computer assisted folding analysis showed that the intergenic region was rich in A and U residues where two distant hairpin structures could be formed. One was highly conserved and stable, but the other was rather unstable because of bases variation. Therefore, there were molecular variation among isolates of rice stripe virus in China.
- Rice stHpe virus
- , RNA4 intergenic regions
- , Sequence comparison

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequence Comparison of Intergenic Region between Two Isolates of Rice Stripe Virus RNA4

1. Institute of Plant Virologyo of Fujian Agricuhurat University ,Key Laboratory of Plant Virology of Fujian Province，Fuzhou 350002,China

Abstract: The RNA4 intergenic regions of two isolates of rice stripe virus (RSV) in China (de signated as RSV JN and RSV YL) were amplified by RT PCR and cloned into pGEM T easy vector. Sequence analysis showed that they were all 654 bp in length, and shared only 92% identity at the nucleotide level. Computer assisted folding analysis showed that the intergenic region was rich in A and U residues where two distant hairpin structures could be formed. One was highly conserved and stable, but the other was rather unstable because of bases variation. Therefore, there were molecular variation among isolates of rice stripe virus in China.