Comparing DNA Sequences in Glycoprotein I Gene of Marek,s Disease Viruses of Different Pathotypes

CHI Zhong; HE Liang-Mei

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April 2000

CHI Zhong and HE Liang-Mei. Comparing DNA Sequences in Glycoprotein I Gene of Marek,s Disease Viruses of Different Pathotypes[J]. Virologica Sinica, 2000, 15(2): 180-187.

Citation: CHI Zhong, HE Liang-Mei. Comparing DNA Sequences in Glycoprotein I Gene of Marek,s Disease Viruses of Different Pathotypes .VIROLOGICA SINICA, 2000, 15(2) : 180-187.

特超强毒型马立克病病毒囊膜糖蛋白I基因序列及与其它致病型毒株的比较

崔治中 ,
何良梅

1.
扬州大学畜牧兽医学院兽医系，扬州225009

摘要

特超强毒型648A株马立克病病毒(MDV)的囊膜糖蛋白I(gI)基因经PCR扩增后克隆进pUC18质粒载体,并对其ORF完成了DNA测序。与已发表的其它致病型毒株的糖蛋白I的DNA和氨基酸序列比较表明,648A株的gI基因序列与超强毒RBIB株已发表的ORF5端761个碱基完全相同。但是在该基因中完整ORF的1068个碱基中,648A株与强毒GA株间有8个碱基变异并导致7个氨基酸的变化,且这一变化主要发生在该基因的5端,其3端三分之一完全相同.
- 马立克病病毒
- , 糖蛋白I基因
- , 序列比较

Comparing DNA Sequences in Glycoprotein I Gene of Marek,s Disease Viruses of Different Pathotypes

CHI Zhong ,
HE Liang-Mei

1.
Dept.of Veterinary Medicine,Yangzhou University， Yangzhou 225009，China

Abstract

The glycoprotein I gene (gI) of vv +MDV strain 648A was amplified by PCR, cloned and sequenced, its DNA and amino acid sequences were compared to published sequences of vMDV strain GA and vvMDV strain RB1B. The results indicated that the gI sequence of 648A was identical to the published 761 bases of 5 side of ORF of strain RBIB, but there were 8 bases and then 7 amino acids different between GA and 648A strains. All these base changes located at the 5 end of gI, about the one third of the 3 end was identical in different pathotypes.
- Marek,s disease virus
- , Glycopm tein I gene
- , DNA sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparing DNA Sequences in Glycoprotein I Gene of Marek,s Disease Viruses of Different Pathotypes

CHI Zhong ,
HE Liang-Mei

1. Dept.of Veterinary Medicine,Yangzhou University， Yangzhou 225009，China

Abstract: The glycoprotein I gene (gI) of vv +MDV strain 648A was amplified by PCR, cloned and sequenced, its DNA and amino acid sequences were compared to published sequences of vMDV strain GA and vvMDV strain RB1B. The results indicated that the gI sequence of 648A was identical to the published 761 bases of 5 side of ORF of strain RBIB, but there were 8 bases and then 7 amino acids different between GA and 648A strains. All these base changes located at the 5 end of gI, about the one third of the 3 end was identical in different pathotypes.