Amplification and Cloning of NS3 Gene of Dengue Virus

JIANG Li-Fang; YAN Hui-Jun; BO Wen-Tong; FANG Dan-Yun; GUO Hui-Yu

PDF
Cite
Share

facebook

twitter

google

linkedin

April 2000

JIANG Li-Fang, YAN Hui-Jun, BO Wen-Tong, FANG Dan-Yun and GUO Hui-Yu. Amplification and Cloning of NS3 Gene of Dengue Virus[J]. Virologica Sinica, 2000, 15(2): 193-196.

Citation: JIANG Li-Fang, YAN Hui-Jun, BO Wen-Tong, FANG Dan-Yun, GUO Hui-Yu. Amplification and Cloning of NS3 Gene of Dengue Virus .VIROLOGICA SINICA, 2000, 15(2) : 193-196.

登革病毒NS3全基因的扩增与克隆

1.
中山医科大学微生物学教研室，广州510089

摘要

登革热(ＤＦ)、登革出血热及登革休克综合征(ＤＨＦ/ＤＳＳ)是由登革病毒所致的两种不同临床类型的急性传染病,广泛流行于全球热带及亚热带地区。ＤＨＦ/ＤＳＳ以高热、出血、休克、高病死率为主要特征,近年来其发病率有迅速增加的趋势,已成为严重影响人类健康的公共卫生...
- 登革病毒NS3基因
- , 逆转录PCR
- , 分子克隆

Amplification and Cloning of NS3 Gene of Dengue Virus

1.
Department of Microbiology,Sue yet-sen University of Medical Siences，Guangzhau 510089,China

Abstract

The RNA of dengue 2 virus (New Guinea C strain, NGC) was extracted from small amount of dengue 2 virus culture supernatant. The large NS3 gene fragment was amplified from RNA of dengue 2 virus with RT PCR method. The amplified NS3 gene fragment was cloned directly into the pUC18 plasmid vector. The recombinant plasmid was identified by agarose gel electrophoresis analysis after digestion with restriction endonuclease and nested PCR method. The results showed that the amplified NS3 gene fragment was about 1978 bp in length; the recombinant plasmid contained NS3 gene of dengue 2 virus. The method of amplified large fragment of dengue virus from small amount of dengue virus culture supernatant was established.
- Dengue virus NS3 gene
- , RT-PCR
- , Molecular cloning

References
Proportional views

PDF

Article Metrics

Article views(4102) PDF downloads(819) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Amplification and Cloning of NS3 Gene of Dengue Virus

1. Department of Microbiology,Sue yet-sen University of Medical Siences，Guangzhau 510089,China

Abstract: The RNA of dengue 2 virus (New Guinea C strain, NGC) was extracted from small amount of dengue 2 virus culture supernatant. The large NS3 gene fragment was amplified from RNA of dengue 2 virus with RT PCR method. The amplified NS3 gene fragment was cloned directly into the pUC18 plasmid vector. The recombinant plasmid was identified by agarose gel electrophoresis analysis after digestion with restriction endonuclease and nested PCR method. The results showed that the amplified NS3 gene fragment was about 1978 bp in length; the recombinant plasmid contained NS3 gene of dengue 2 virus. The method of amplified large fragment of dengue virus from small amount of dengue virus culture supernatant was established.