Construction of Enkaryotie Expression Plasm ids of CSFV E2 Gene and the Study on DNA Vaccine

TU Xin-Long; CHU Chang-Chun; LI Gong-Wei; CHEN Chuang-Fu; LI Zuo-Sheng; MA Zheng-Hai; SUN Meng; YAN Shen-

PDF
Cite
Share

facebook

twitter

google

linkedin

June 2000

TU Xin-Long, CHU Chang-Chun, LI Gong-Wei, CHEN Chuang-Fu, LI Zuo-Sheng, MA Zheng-Hai, SUN Meng and YAN Shen-. Construction of Enkaryotie Expression Plasm ids of CSFV E2 Gene and the Study on DNA Vaccine[J]. Virologica Sinica, 2000, 15(3): 264-271.

Citation: TU Xin-Long, CHU Chang-Chun, LI Gong-Wei, CHEN Chuang-Fu, LI Zuo-Sheng, MA Zheng-Hai, SUN Meng, YAN Shen-. Construction of Enkaryotie Expression Plasm ids of CSFV E2 Gene and the Study on DNA Vaccine .VIROLOGICA SINICA, 2000, 15(3) : 264-271.

猪瘟病毒E2基因真核表达质粒的构建及基因疫苗的研究

1.
解放军农牧大学军事兽医研究所,长春130062

摘要

构建了猪瘟病毒 (classicalswinefevervirus,CSFV)主要保护性抗原E2基因 4种不同的真核表达质粒。小鼠免疫试验表明 ,E2基因上不同的功能区对基因疫苗的免疫应答有很大影响 ,有信号肽序列的E2基因可诱导产生特异性免疫反应 ,且无跨膜区序列的E2基因所诱导的免疫应答反应比有跨膜区序列的强 ,而无信号肽序列的E2基因则不能诱导产生CSFV特异性的免疫反应。攻毒保护试验表明 ,免疫家兔最少可抵抗 10个最小感染剂量 (MID)的猪瘟兔化弱毒苗 (Hogcholeralap inizedvirus,HCLV)的攻击 ;免疫猪可抵抗致死剂量的CSFV石门株强毒的攻击。
- 猪瘟病毒
- , E2基因
- , 真核表达质粒
- , 基因疫苗

Construction of Enkaryotie Expression Plasm ids of CSFV E2 Gene and the Study on DNA Vaccine

1.
Changchun University of Agricultural andAnimal Science,Changchun 1300621, China

Abstract

Four kinds of eukaryotic expressing plasmids of classical swine fever virus (CSFV) E2 gene, either containing or lacking the signal sequence and transmembrane region sequence, were constructed. The antibodies against CSFV were induced after immunization of mice by intramuscular (IM) injection with the plasmids of E2 gene with a signal sequence, pcDST and pcDSW, but not with the plasmids of E2 gene lacking a signal sequence, pcDWT and pcDWW. The level of antibodies elicited by pcDSW lacking transmembrane region sequence was higher than that elicited by pcDST with transmembrane region sequence. The results indicated the plasmid expressing a secreted antigen may be more efficient for induction of immune response when administered by IM injection than that expressing non secreted antigen. Immunization with pcDSW could protect rabbits against challenge with at least 10 MID hog cholera lapinized virus (HCLV) and protect pig from the challenge of lethal dose of CSFV strain, Shimenmen.
- CSFV
- , E2 gene
- , Eukaryotic expression
- , Plasmid

References
Proportional views

PDF

Article Metrics

Article views(4459) PDF downloads(960) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of Enkaryotie Expression Plasm ids of CSFV E2 Gene and the Study on DNA Vaccine

1. Changchun University of Agricultural andAnimal Science,Changchun 1300621, China

Abstract: Four kinds of eukaryotic expressing plasmids of classical swine fever virus (CSFV) E2 gene, either containing or lacking the signal sequence and transmembrane region sequence, were constructed. The antibodies against CSFV were induced after immunization of mice by intramuscular (IM) injection with the plasmids of E2 gene with a signal sequence, pcDST and pcDSW, but not with the plasmids of E2 gene lacking a signal sequence, pcDWT and pcDWW. The level of antibodies elicited by pcDSW lacking transmembrane region sequence was higher than that elicited by pcDST with transmembrane region sequence. The results indicated the plasmid expressing a secreted antigen may be more efficient for induction of immune response when administered by IM injection than that expressing non secreted antigen. Immunization with pcDSW could protect rabbits against challenge with at least 10 MID hog cholera lapinized virus (HCLV) and protect pig from the challenge of lethal dose of CSFV strain, Shimenmen.