DAN Zheng-Li, Durand Stephanie and Bonami Jean-Robert. Screening of DNA Polymerase Gene from W hite Spot Syndrome Virus (WSSV)by Using Degenerated Oligonueleotide[J]. Virologica Sinica, 2000, 15(3): 302-307.
Citation: DAN Zheng-Li, Durand Stephanie, Bonami Jean-Robert. Screening of DNA Polymerase Gene from W hite Spot Syndrome Virus (WSSV)by Using Degenerated Oligonueleotide .VIROLOGICA SINICA, 2000, 15(3) : 302-307.

用简并寡核苷酸探针筛选对虾白斑综合征病毒DNA多聚酶基因片段

  • 根据一些病毒的DNA多聚酶氨基酸序列中特有的保守序列VYGDTD设计的简并寡核苷酸 ,经地高辛标记后与对虾白斑综合征病毒基因库克隆杂交 ,筛选出一段长度为 70 7bp的EcoRI基因片段 ,该片段在一个开放阅读框内。并含DNA多聚酶B家族特有的保守序列YGDTDS。经与基因库比较 ,其氨基酸序列与藻类DNA病毒科 (Phycodnaviridae)的几株藻类病毒的DNA多聚酶片段有部分相似 ,因此推测该核苷酸片段为对虾白斑综合征病毒DNA多聚酶基因的部分序列。

Screening of DNA Polymerase Gene from W hite Spot Syndrome Virus (WSSV)by Using Degenerated Oligonueleotide

  • Degenerated oligonucleotides were designed from one motif VYGDTDS of several viral DNA polymerases and were labelled with Digoxigenin to screen DNA polymerase gene in a White Spot Syndrome Virus (WSSV) DNA library. One Eco R I fragment of 707 bp was selected and contains a putative open reading frame in which the motif YGDTDS of family B of DNA polymerase was found. This selected sequence was subsequently compared with GenBank data. The results showed that this deduced amino acids sequence has some similarities with several algal like virus of the family Phycodnavirus. So we deduced that this is one DNA polymerase gene fragment of WSSV.

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    Screening of DNA Polymerase Gene from W hite Spot Syndrome Virus (WSSV)by Using Degenerated Oligonueleotide

    • 1. Wulmn Institute of Virology,Chinese Academy of Sciences,Wahan 43007[,CAina

    Abstract: Degenerated oligonucleotides were designed from one motif VYGDTDS of several viral DNA polymerases and were labelled with Digoxigenin to screen DNA polymerase gene in a White Spot Syndrome Virus (WSSV) DNA library. One Eco R I fragment of 707 bp was selected and contains a putative open reading frame in which the motif YGDTDS of family B of DNA polymerase was found. This selected sequence was subsequently compared with GenBank data. The results showed that this deduced amino acids sequence has some similarities with several algal like virus of the family Phycodnavirus. So we deduced that this is one DNA polymerase gene fragment of WSSV.

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