Expression of Full-length HCV NS3 Gene in E ．coli and Its Application in HCV Antibody Detection

ZHANG Bin; XIE Lin-Bai; GAO Jin-Rong; XU Jin-Beng; RUAN Hua; WANG Xiao-Ling; DIAO Ru-E

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June 2000

ZHANG Bin, XIE Lin-Bai, GAO Jin-Rong, XU Jin-Beng, RUAN Hua, WANG Xiao-Ling and DIAO Ru-E. Expression of Full-length HCV NS3 Gene in E ．coli and Its Application in HCV Antibody Detection[J]. Virologica Sinica, 2000, 15(3): 308-311.

Citation: ZHANG Bin, XIE Lin-Bai, GAO Jin-Rong, XU Jin-Beng, RUAN Hua, WANG Xiao-Ling, DIAO Ru-E. Expression of Full-length HCV NS3 Gene in E ．coli and Its Application in HCV Antibody Detection .VIROLOGICA SINICA, 2000, 15(3) : 308-311.

HCV全长NS3基因表达及在抗体检测中的应用

1.
武汉大学病毒学研究所，武汉430072

摘要

丙型肝炎病毒 (ＨＣＶ)是引起非甲非乙型肝炎的主要病原因子。被ＨＣＶ感染的病例中 ,超过 5 0 %以上会引起持续性感染、慢性肝炎 ,最终可能引起肝硬化和肝细胞癌[1] 。ＨＣＶ严重威胁人类健康 ,但目前对丙肝患者尚缺乏有效的治疗手段 ,因此 ,严格把好血源关 ,提高对丙肝?..
- 丙型肝炎病毒
- , NS3 表达
- , 抗体检测

Expression of Full-length HCV NS3 Gene in E ．coli and Its Application in HCV Antibody Detection

1.
Institute of Virology， Wuhan University，Wuhan 430072，China

Abstract

Full length NS3 gene of a hepatitis C virus was amplified by PCR using plasmid pBAC25 containing HCV nonstructural protein gene as template. The amplified fragment (about 1.8 kb) was cloned into plasmid pQE30 and the recombinant plasmid was expressed in JM109. The NS3 protein was purified by NiSO 4 metal chelating resin, and its antigenecity was determined by ELISA, the results showed that the full length NS3 protein was more sensitive than the commercial carboxy terminal domain of NS3 protein in HCV antibody detection.
- Hepatitis C virus
- , NS3
- , Expression

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Expression of Full-length HCV NS3 Gene in E ．coli and Its Application in HCV Antibody Detection

1. Institute of Virology， Wuhan University，Wuhan 430072，China

Abstract: Full length NS3 gene of a hepatitis C virus was amplified by PCR using plasmid pBAC25 containing HCV nonstructural protein gene as template. The amplified fragment (about 1.8 kb) was cloned into plasmid pQE30 and the recombinant plasmid was expressed in JM109. The NS3 protein was purified by NiSO 4 metal chelating resin, and its antigenecity was determined by ELISA, the results showed that the full length NS3 protein was more sensitive than the commercial carboxy terminal domain of NS3 protein in HCV antibody detection.