Preliminary Study on the Activation of TRAFs M ediated by Epstein-Barr Virus Encoded LM P1 in Nasopharyngeal Carcinoma

WANG Cheng—xing; LI Xiao—yan; GU Huan—hui; DENG Xu-yun; CAO Ya

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February 2001

WANG Cheng—xing, LI Xiao—yan, GU Huan—hui, DENG Xu-yun and CAO Ya. Preliminary Study on the Activation of TRAFs M ediated by Epstein-Barr Virus Encoded LM P1 in Nasopharyngeal Carcinoma[J]. Virologica Sinica, 2001, 16(1): 6-10.

Citation: WANG Cheng—xing, LI Xiao—yan, GU Huan—hui, DENG Xu-yun, CAO Ya. Preliminary Study on the Activation of TRAFs M ediated by Epstein-Barr Virus Encoded LM P1 in Nasopharyngeal Carcinoma .VIROLOGICA SINICA, 2001, 16(1) : 6-10.

鼻咽癌中EB病毒LMP1激活TRAFs韵初步研究

1.
湖南医科大学肿宿研究所，长沙 410078

摘要

在EB病毒潜伏膜蛋白LMP1介导的信号传导通路中．TRAFs作为LMP1活化的第一位信号分子，可能扮演着重要的分子开关佑也令^关注的是，在上皮性肿瘤NPC的发生中，EB病毒LMP1髓否激活重要的TRAFs情号舒子‘ 究竟激活何补 I’RAFs信号分子，激活的机制何在?将LMP1 eDNA导八LMP1表达阴性的HNE2中．建立稳定表达I MP1的鼻啊螂细胞系：HNE2．I MPI。以此为材料．应用差异RT—PCR和We,rern blottirtg法证实，无沧在RNA水’ 迁址 I 『水平J ．TRAF1在HNE2一I．MP1中表达鞍HNE2强．而TRAF2及TRAF3在HNE2． I，MP1 HNE2细咆【{1 然止明显差异；进⋯步用免疫共沉淀一We．stern blotting证实LMP1可使TRAFI、TRAF2 I’RAF3磷酸ILi~i设活1t 选些结果提示在鼻咽癌中．I MP1可能诱导TRAF1表述，而对TRAF2段TRAF3并不影响．但[．MPI Tif磷睦化 rRAF1、TRAF2、TRAF3而使其功能性活化。
- 鼻咽癌
- , 潜伏膜蛋白
- , 肿增坏死因子受体相关固子：信号传导

Preliminary Study on the Activation of TRAFs M ediated by Epstein-Barr Virus Encoded LM P1 in Nasopharyngeal Carcinoma

1.
Cancer Research Institute，Hunan Medical University，Changsha 410078 China

Abstract

The present is aimed at identifying the activation of TRAFs in nasopharyngeal carcinoma (NPC)ill"oitro The differentia1 expression of TRAF2、TRAF3 was not detected in RNA and protein 1evel，whereas the expression of TRAF1 in HNE2一LM P1 eel1 1ines was much more abundant than that in HNE2 cell lines， suggesting that TRAF1 niay be an inducible molecule： More importantly． TRAF1，TRAF2 or TRAF3 were activated in the HNE2一LMP1 cells，whereas TRAF1，TRAF2 or TRAF3 were not activated in HNE2 cells as detected by the immunoprecipitation-W estern blotting as— say These data provide an experimental basis for our study beginning from the signal transducation pathway for the eluccidation of the mechanism of LM P1 carcinogenesis in NPC
- Nampharyngeal carcinoma
- , Latent mem brane protein 1：Tumor necrosis factor receptor associated [actors
- , Signal transducation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preliminary Study on the Activation of TRAFs M ediated by Epstein-Barr Virus Encoded LM P1 in Nasopharyngeal Carcinoma

1. Cancer Research Institute，Hunan Medical University，Changsha 410078 China

Abstract: The present is aimed at identifying the activation of TRAFs in nasopharyngeal carcinoma (NPC)ill"oitro The differentia1 expression of TRAF2、TRAF3 was not detected in RNA and protein 1evel，whereas the expression of TRAF1 in HNE2一LM P1 eel1 1ines was much more abundant than that in HNE2 cell lines， suggesting that TRAF1 niay be an inducible molecule： More importantly． TRAF1，TRAF2 or TRAF3 were activated in the HNE2一LMP1 cells，whereas TRAF1，TRAF2 or TRAF3 were not activated in HNE2 cells as detected by the immunoprecipitation-W estern blotting as— say These data provide an experimental basis for our study beginning from the signal transducation pathway for the eluccidation of the mechanism of LM P1 carcinogenesis in NPC