Molecular Cloning and Nucleotide Sequence Analysis of the M Genome Segment of Hantavirus Z37，a Chinese HFRS Vaccine Strain

ZHU Han-ping; LIU He—bin; YAO Ping·ping; ZHU Zhi—yong

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February 2001

ZHU Han-ping, LIU He—bin, YAO Ping·ping and ZHU Zhi—yong. Molecular Cloning and Nucleotide Sequence Analysis of the M Genome Segment of Hantavirus Z37，a Chinese HFRS Vaccine Strain[J]. Virologica Sinica, 2001, 16(1): 15-21.

Citation: ZHU Han-ping, LIU He—bin, YAO Ping·ping, ZHU Zhi—yong. Molecular Cloning and Nucleotide Sequence Analysis of the M Genome Segment of Hantavirus Z37，a Chinese HFRS Vaccine Strain .VIROLOGICA SINICA, 2001, 16(1) : 15-21.

汉坦病毒中国疫苗株Z37M 片段的克隆及序列分析

1.
浙江省疾病预防控制中心．肾综合征出血热重点实验室，浙江杭州市310009

摘要

汉坦病毒7-37株足从榻家鼠体内分离到的，用于生产取价肾综合征出血热疰苗的病毒毒株之一，血清分型为 SEO型利用RT-PCR ‘法扩增Z37株M 基因片段eDNA，克隆入质粒载体，进行核苷酸序列测定及分析。Z37 株M基固片段由3651十椅苷酸组成，只有一十开放读码框架，共编码1133个氨基酸。与HTN型病毒(76 118、 A9、HV一114)的核苷酸干¨氰基酸同源性分别为71 8％～72 1％、76 2％～76 7％，与SEO型(R22、L99、80—39)的核苷醍和氮基酸同源性讣为95 3％～96 1％、95 3％～98 9％这一结果的获得进一步从分子水平确定了Z37株的型别，井为研制M肇段重组疫苗打下基础。
- 汉坦病毒
- , Z37株
- , M片段
- , 序列测定

Molecular Cloning and Nucleotide Sequence Analysis of the M Genome Segment of Hantavirus Z37，a Chinese HFRS Vaccine Strain

1.
Zhejiang Provincial Key laboratory of HFRS， Zhejiang Provincial Center Disease Prevention and Control，Hangzhou 310009，China

Abstract

0ne of the strain of bivalent HFRS vaccine．Z37 strain was isolated from Rattus norvegicus and identified aS SEO virus by serological test The M segment cDNA of Hantavirus Z37 strain WaS obtained by reverse transcription and polymerase chain reaction，subsequently cloned into pGEM—T vector．The sequence of positive recombinants was determined by the method of dideoxy chain term[一 nation，which revea led that the M genomic segment is 3651 nucleotide in length with a predicated bng open reading frame encoding a protein of 1133 amino acids Comparison with HNT type(76—118．A9， HV一114 strains)indicatedthattherewere 71 8％～72．1％ homolng y atthe nucleotideleve1．76．2～ 76．7％ homology at the amino acid leve1．Co mparison with SEO type (R22，L99．80-39 strains) showed 95．3～ 96 l homology at the nucleotide leve1．95 3～99．2％ homology at the amino acid 1ev d The results of nucleotide and amino acid comparison indicated that Z37 strain is SEO viruses in molecular Ieve 1．
- Hantavirus
- , Z37 strain
- , M genome segment
- , Sequencing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Molecular Cloning and Nucleotide Sequence Analysis of the M Genome Segment of Hantavirus Z37，a Chinese HFRS Vaccine Strain

1. Zhejiang Provincial Key laboratory of HFRS， Zhejiang Provincial Center Disease Prevention and Control，Hangzhou 310009，China

Abstract: 0ne of the strain of bivalent HFRS vaccine．Z37 strain was isolated from Rattus norvegicus and identified aS SEO virus by serological test The M segment cDNA of Hantavirus Z37 strain WaS obtained by reverse transcription and polymerase chain reaction，subsequently cloned into pGEM—T vector．The sequence of positive recombinants was determined by the method of dideoxy chain term[一 nation，which revea led that the M genomic segment is 3651 nucleotide in length with a predicated bng open reading frame encoding a protein of 1133 amino acids Comparison with HNT type(76—118．A9， HV一114 strains)indicatedthattherewere 71 8％～72．1％ homolng y atthe nucleotideleve1．76．2～ 76．7％ homology at the amino acid leve1．Co mparison with SEO type (R22，L99．80-39 strains) showed 95．3～ 96 l homology at the nucleotide leve1．95 3～99．2％ homology at the amino acid 1ev d The results of nucleotide and amino acid comparison indicated that Z37 strain is SEO viruses in molecular Ieve 1．