CHEN De, PAN Jie-yan, JIA Liqun, CAI Bao-xiang and CHEN Puyan. Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu,China[J]. Virologica Sinica, 2001, 16(1): 22-27.
Citation: CHEN De, PAN Jie-yan, JIA Liqun, CAI Bao-xiang, CHEN Puyan. Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu,China .VIROLOGICA SINICA, 2001, 16(1) : 22-27.

传染性支气管炎病毒江苏分离株核蛋白基因序列测定与同源性分析

  • 将本室鸡传染性正气管毙病毒(IBV)江苏省地方分离肾型毒株Js/95/03接种鸡胚.分离、纯化病毒。提取单 股RNA做为反转录.聚台酶链反应(RT—PCR)的扩增模板。用Genhank公开序列多重比较后设计一对引物,使用 单管RT—PCR方法,特 扩增IBV核蛋白(N)基因5‘端854 bp的片段。扩增产物纯化后测序.序列分折表明.IBV N基因也存在较大变异.此毒株与呼吸型疫苗株M41序列同源性最高。

Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu,China

  • Field avian infectious bronchitis virus(IBV)designated as JS/95/o3。which was isolated from Jiangsu province of china,was cultivated in chicken embryo.It’S single strain RNA was extract— ed from purified virus and worked as template of reverse transcription polymexase chain reaction f RT— PCR),a pair of primer designed according to megalign results of published IBV sequences in Genbank was used to amplify the neucleocapsid gene,the RT·PCR product was sequenced directly Sequence analysis revealed that the sequence of JS/95/03 is most homologized with that of M41 strain

  • 加载中
  • 加载中

Article Metrics

Article views(3792) PDF downloads(806) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu,China

    • 1. 1 Key laboratory of animal disease diagnosticⅡ immun~ogy.Ministry of Agriculture, Nanjing agricultural university.Nanjing 210095,China

    Abstract: Field avian infectious bronchitis virus(IBV)designated as JS/95/o3。which was isolated from Jiangsu province of china,was cultivated in chicken embryo.It’S single strain RNA was extract— ed from purified virus and worked as template of reverse transcription polymexase chain reaction f RT— PCR),a pair of primer designed according to megalign results of published IBV sequences in Genbank was used to amplify the neucleocapsid gene,the RT·PCR product was sequenced directly Sequence analysis revealed that the sequence of JS/95/03 is most homologized with that of M41 strain

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return