W ANG Youchun。, ZHANG Hua—yuan, GU Wen—jie .Roger Ling .LI He-min。.Tim J Harrison and Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV)[J]. Virologica Sinica, 2001, 16(1): 28-33.
Citation: W ANG Youchun。, ZHANG Hua—yuan, GU Wen—jie .Roger Ling .LI He-min。.Tim J Harrison, . Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV) .VIROLOGICA SINICA, 2001, 16(1) : 28-33.

新型戊肝病毒ORF3和部分ORF1基因的序列分析

  • 用RT-PCR方法对2例感染新型HEV的样品进行了检测,井对PCR产物进行了克隆及测序。捡测结果显示用 常规PCR检谢易造成ORF3中GC丰富区的缺失、但在常规PCR反应液中加入GC mdt溶液可成功地扩增GC丰富 匡 序列分析显示_r1种I TI1属于同一基因型、但不同于巳报道的HEV l型、ll型和l【I型.为一新的基因型 T1和 T11与 在该区的核f 酸同源性为79%~82%;与l【型的同源性为8【I%-81%;和lIl型的同源性为83%-85%

Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV)

  • ORF3 and partial ORF1 regions were amplified with RT-PCR from ta~vo patients(T1 and TII)infected with new genotype of hepatitis E Virus.The PCR products were cloned and sequenced The results showed that G-C rich region in ORF3 was deleted when amplified with norrflal PCR reac— tion However.PCR reaction containing G-C melt solution can overcome this problem The sequence analysis showed that T1 and Tll belong tO a new genotype of HEV which differs from genotype I,II and III reported.T1 and T11 have 79% -82% .80% -81% and 83% ~85% identical tO genotype I. II and Ill respectively

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    Sequence Analysis of ORF3 and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis E Virus(HEV)

    • 1. 1 Natiottal Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050.China
    • 2. Center for Hepatology,Royal Free Campus,University College London,Hamstead.London NW3 2PF,UK

    Abstract: ORF3 and partial ORF1 regions were amplified with RT-PCR from ta~vo patients(T1 and TII)infected with new genotype of hepatitis E Virus.The PCR products were cloned and sequenced The results showed that G-C rich region in ORF3 was deleted when amplified with norrflal PCR reac— tion However.PCR reaction containing G-C melt solution can overcome this problem The sequence analysis showed that T1 and Tll belong tO a new genotype of HEV which differs from genotype I,II and III reported.T1 and T11 have 79% -82% .80% -81% and 83% ~85% identical tO genotype I. II and Ill respectively

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