HCVE2区基因的分子克隆及序列分析

ZHANG Dongwei; LIAN G; QI Zi; LING Shi

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Volume 16 Issue 1

February 2001

ZHANG Dongwei, LIAN G, QI Zi and LING Shi. HCVE2区基因的分子克隆及序列分析[J]. Virologica Sinica, 2001, 16(1): 40-44.

Citation: ZHANG Dongwei, LIAN G, QI Zi, LING Shi. HCVE2区基因的分子克隆及序列分析 .VIROLOGICA SINICA, 2001, 16(1) : 40-44.

HCVE2区基因的分子克隆及序列分析

张东伟梁布锋祁自柏凌世淦 ,

1.
中国科学院武汉病毒研究所中国药品生物制品检定所军事医学科学院

摘要

用RT PCRKIT从西安血站大样抗HCV阳性血清中筛选出HCVRNA阳性血清 ,提取HCV的RNA ,利用随机引物反转录合成其cDNA并进行半巢式PCR反应。将纯化的PCR产物酶切后与表达载体PET 2 2b+连接 ,经过双脱氧末端终止法双向测序 ,得到 85 2bp长的核苷酸序列。通过将该序列与已知不同型的HCVE2序列比较得知 ,此序列正是HCVⅡ型目的基因
- 丙型肝炎病毒
- , E2包膜糖蛋白
- , HVR1
- , 序列特征

HCVE2区基因的分子克隆及序列分析

1.
1 Wuhan Institute of Virology，Academia Sinica，Wuhan 430071．China’ 2 1p bf Hepatitis．,National Institutefor the Control ofPharmaceutical and Biology Produc~，8dj~g 100050．China

Abstract

HCV RNA positive serum was first selected by RT PCR test kit from several anti HCV positive sera obtained from Xi’an.HCV RNA extracted from the elected sera was converted to cDNA by reverse transcription with random primer.Half nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET 22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates reported previously showed that the sequence belonged to HCV type Ⅱ.
- Hepatitis C Virus E2 glycoprotein HVR1 Sequence character

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

HCVE2区基因的分子克隆及序列分析

1. 1 Wuhan Institute of Virology，Academia Sinica，Wuhan 430071．China’ 2 1p bf Hepatitis．,National Institutefor the Control ofPharmaceutical and Biology Produc~，8dj~g 100050．China

Abstract: HCV RNA positive serum was first selected by RT PCR test kit from several anti HCV positive sera obtained from Xi’an.HCV RNA extracted from the elected sera was converted to cDNA by reverse transcription with random primer.Half nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET 22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates reported previously showed that the sequence belonged to HCV type Ⅱ.