LI Li.jun.ZHOU Zhong.ju.XIE Lian-hui, Cloning of 3’-terminal Part of Sugarcane M osaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV[J]. Virologica Sinica, 2001, 16(1): 45-50.
Citation: LI Li.jun.ZHOU Zhong.ju.XIE Lian-hui, . Cloning of 3’-terminal Part of Sugarcane M osaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV .VIROLOGICA SINICA, 2001, 16(1) : 45-50.

甘蔗花叶病毒3’末端基因的克隆及外壳蛋白序列分析比较

  • 选取我国SCMV化势株系A株系的分离物sci~v.CA为材料,经过病毒和病毒RNA的提纯.反转录获得病 毒cDNA,并克隆到载体pUCI9的Sma l位点上,筛选得到多个重组质粒。选取其中一个克隆SCMV·CA54进行 测序.得到一个垒长为1 296 bp的核苷酸序列。这段序列由一个长为1 O44 bp的开放阅读框架(ORF)和一个长279 bp的3。末端非编码区序列(3’.UTR)及poly(A)尾巴组成。这个ORF包括病毒完整的外壳蛋白(cP)及部分核内 含体蛋白b(NIb)基因序硎。将所得序列同已知SCMV亚组中各株系分离物的核苷酸和氨基酸进行同源性比较. 结果表明该序列与其它株系分离物cP核苷酸序列的同源性介于63.7%~77.6%之同,氨基酸的同源性介于64% ~ 89%之间。根据马特薯Y病毒属的序列同源性划分标准,SCMV.CA与其它株系或分离物的同源性关系均舟于 种与株系划分标准之间。这是我国首次报道SCMV CP基因序列。

Cloning of 3’-terminal Part of Sugarcane M osaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV

  • ScMV.CA,an isolate of the doraJnant A strain in China continent was propagated on sweet maize and purified.Cloned cDNAs representing more than 0.8kb of the 3’termini were obtained after a reverse transcription and ligation to pUC19 in Sma1 site using an ollgo(dT)primer One clone na med SCMV.CA54 was sequenced and the 1ength of the SCMV—CA54 was 1296 bp.The sequenee covered the 3’untranslated region(3’UTR).coat protein(cP)mad part of the nuclear inclusion b (NIb)genes of the virus.The nucleotide and deduced amino acid~equenee in the CP gene hatweell Chinese isolate SCMV.CA and other isolates or strains of SCMV subgroup were compa red.Results showed that the homology of nucleotide sequence in CP gene between SCMV—CA and other isolates or strains of SCMV subgroup ranged from 63.7% to 77.6% .and amino acid sequence from 64% to 89% .This homology degree lies betwen the range for distinct viruses and tha t betwen related strains of potyviruses,arguing tha t the Chinese isolate might represent a distinct virus.

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    Cloning of 3’-terminal Part of Sugarcane M osaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV

    • 1. Institute orPlant Virology ofFujian Agricultural andForestryUniversity.Fuzhou 350002,China

    Abstract: ScMV.CA,an isolate of the doraJnant A strain in China continent was propagated on sweet maize and purified.Cloned cDNAs representing more than 0.8kb of the 3’termini were obtained after a reverse transcription and ligation to pUC19 in Sma1 site using an ollgo(dT)primer One clone na med SCMV.CA54 was sequenced and the 1ength of the SCMV—CA54 was 1296 bp.The sequenee covered the 3’untranslated region(3’UTR).coat protein(cP)mad part of the nuclear inclusion b (NIb)genes of the virus.The nucleotide and deduced amino acid~equenee in the CP gene hatweell Chinese isolate SCMV.CA and other isolates or strains of SCMV subgroup were compa red.Results showed that the homology of nucleotide sequence in CP gene between SCMV—CA and other isolates or strains of SCMV subgroup ranged from 63.7% to 77.6% .and amino acid sequence from 64% to 89% .This homology degree lies betwen the range for distinct viruses and tha t betwen related strains of potyviruses,arguing tha t the Chinese isolate might represent a distinct virus.

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