1日龄非免疫鸡分5jII接种马立克氏病病毒(MDV)I型强毒GA抹、I型MDV疫苗毒CVI988株和llJ型火鸡疱疹 病毒(}ⅣT)疫苗抹后第4 H起．定期采血并用抗MDV囊膜塘蛋白B(gB)单克隆抗体舟导的间接免疫荧光试验检测 MDV在外同血液单棱细胞(pBMCs)中的感染状况。结果发现．自接种【型强毒GA抹后第4日到鸡发病死亡前。都 能检出GA株引起的病群皿症．并于2周左右达到高峰；白接种CVI988株后第4日到第20日止．能检出病毒血症。并 于第8天左右达到高峰； 接种}ⅣT后第4日到第16日止．能检出病毒血症．并于第6天左右达到高峰。与此同时． 将GA株病毒血症的IFA舱}91I结果与细胞培养上病毒空斑计数试验结果比较。发现IFA试验比空斑计数试验更为敏 感。本试验既可用于翔断对鸡作MDV疫苗免疫的接种效果．叉可用于检涮MDV野毒感染状态。

Abstract：One day-old unvaccinated chicks were inoculated with serotype I virulent strain GA and vac— cine strain CVI988．semtype III vaccine strain HVT of Marek’s disease viruses(MDV)．By using an indirect immunofluorescence assay of suspensions of peripheral blood mononuclear 0e11s(PBMC)with monoclonal antibodies(MAb)BA4 and B specific to glycoprotein B of MDV，The dynamic courses of viremia levels for each of 3 strains from 4 days to 45 days postinfection(PI)were determind．Viremia f0r virulent strain GA—infected chiekens were detected from 4 days PI untiI days before death of infect— ed chickens．it got its peak at about 14 days PI．Viremm in serotype 1 vaccine CVI988一inoculated chickensweremeasured between 4t022 days PI．it couldbe detectedfrom 4 days PIan d ended at18 days PI，it got its peak at 8 days PI．For serotype 3 vaccine HVT-inoculated chickens，viremia could be detected beween 4 to 14 days PI，its peak happened at about 6 days PI．Such IFA with MAbs BA4 and B could be used r。evaluate quality of vaccination process in chicken flocks．Tbe differentiM dy— namie courses of viremia for different strains may be used t0 diagnose virulent V infection in vacci— hated flocks．The viremia levels measured by IFA with the MAbs an d co-cultivation assay for plague forming unit(PFU)were compared．The results indicated that IFA assay of PBMC suspension was more sensitive and specific than co—cultivation assay．i．e．viremia levels measured by IFA were 30 t0 100一fold bigher than that by plague—form ing assay in CEF for the same sampie