Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus

QIN Ai; CUI Zhi; Lucy Lee; Aly Fadly

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February 2001

QIN Ai, CUI Zhi, Lucy Lee and Aly Fadly. Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus[J]. Virologica Sinica, 2001, 16(1): 68-73.

Citation: QIN Ai, CUI Zhi, Lucy Lee, Aly Fadly. Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus .VIROLOGICA SINICA, 2001, 16(1) : 68-73.

禽白血病病毒J亚群env基因的克隆和序列分析

秦爱建 ,
崔治中 ,

1.
I扬州大学兽医系，江苏扬州225009
2.
Avian disease and oncotogy laboratory．USDA，East I M ng，MI 48823

摘要

摘要：应用多聚酶链反心(PCR)的方法扩增出ADOI．．4817毒株的囊膜蛋白帆基因，并克隆进大酾杆菌。经核酸序列分析明 t，艇⋯的人小为1 746 bp 其中gp85和gp37由1 554 bp组成，可翻译成517个氨基酸，分子量为 57 7 kD 橄据精晕化他 N X S／T的特点，发现ADO1，4817的删蛋白有15个潜在的糖基化位点。同源性分析证明．ADOI，4817 ，与其它Al v_J的e 基因序列同源性为88 8％～92 4％，而与外源性Al vs的相应序列的同源性仅为40 5％～51 4％，然而．与内源性的EAV—HP毒株的类帆基因的同源性高达91 2％；另外， ADO[ ．48I7毒株的gp37 C末端多了l3十氨基酸这些结果提示，AI．v．J的eFt~,基因存在广泛的变异性，基l 可能来源于内源~rl!f1]外性AI Vs的重组。
- 禽白血病病毒
- , 囊膜蛋白基因
- , 克隆
- , 定序

Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus

1.
1．Dept of Veterinary Medicine， Yangzhou Uniwersity，Yangzhou 225009·China 2 Avian disease and ontology laboratory．USDA．East lansing，MI 48823 USA

Abstract

The envelope gene of ADOL-4817 strain of avian leukosis v[ros subgroup J(ALV—J)was amplified by polymerase chain reaction(PCR)and cloned into TA vector．The sequence analysis re— suits showed that tbe envelope gene is composed of 1 746 bp．1 554 bp of which could be translated in— to 517 amino acids for gp85 and gp37．The molecular weight of envelope protein is 57．7kD There are I5 potential glycosy[ation sites in the envelope protein，13 of which is located in gp85，Analysis of se— quences of envelope gene indicate that ADOL-48 17 showed high degree of sequence identity to other ALV—J strains，and most closely related to the like—envelope gene of endogenous virus EAV—HP but di— vergent from these of other ALV subgroup A-E ．These data support the hypothesis that envelope gene of avian leukosis virus suhgroup J maybe acquired hy l：ecombination with expressed sequences
- Avian 1．eukosls Virus
- , Envelope Gene
- , Cloning
- , Sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequencing of Envelope Gene of Subgroup J Avian Lenkosis Virus

1. 1．Dept of Veterinary Medicine， Yangzhou Uniwersity，Yangzhou 225009·China 2 Avian disease and ontology laboratory．USDA．East lansing，MI 48823 USA

Abstract: The envelope gene of ADOL-4817 strain of avian leukosis v[ros subgroup J(ALV—J)was amplified by polymerase chain reaction(PCR)and cloned into TA vector．The sequence analysis re— suits showed that tbe envelope gene is composed of 1 746 bp．1 554 bp of which could be translated in— to 517 amino acids for gp85 and gp37．The molecular weight of envelope protein is 57．7kD There are I5 potential glycosy[ation sites in the envelope protein，13 of which is located in gp85，Analysis of se— quences of envelope gene indicate that ADOL-48 17 showed high degree of sequence identity to other ALV—J strains，and most closely related to the like—envelope gene of endogenous virus EAV—HP but di— vergent from these of other ALV subgroup A-E ．These data support the hypothesis that envelope gene of avian leukosis virus suhgroup J maybe acquired hy l：ecombination with expressed sequences