ZHANG Hai, ZHANG Yun-zhi, HUANG W, MI Zhu, GONG He-qin and WANG Jing. Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province[J]. Virologica Sinica, 2001, 16(1): 74-77.
Citation: ZHANG Hai, ZHANG Yun-zhi, HUANG W, MI Zhu, GONG He-qin, WANG Jing. Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province .VIROLOGICA SINICA, 2001, 16(1) : 74-77.

从云南省蝙蝠脑组织中分离出乙型脑炎病毒

  • 摘要:为进一步阐明蝙螭 保存乙齄病毒中的作用.于1997年7月.在云南省耿马县捕捉蝙蝠64其,取齄组织作病 毒分离.从一只盒管鼻蜗脑组织中分离出1株病毒。该毒株能引起BHK21细胞病变和乳鼠发病死亡.在pH5 75~ 7.4对能凝集鸽红血球.疑用单克隆抗体血凝抑制和免疫荧光试验鉴定,证实为乙型脑炎病毒。进一步证明蝙蝠在 己型脑兜病毒保存和扩敬中具有重要作用。从金管鼻蝠体内分离出乙型脑炎病毒届国内外首次报遭。

Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province

  • In July 1997, a strain (GB30) of virus was isolated from 60 samples of brain tissues of Murina aurata (Chiroptera: Vespertilionidae) collected in Gengma county, Yunnan province, China. Isolation of virus was negative from 4 samples of brain tissues of Rousettus leschenaulti (Chiroptera: Pteropodidae) collected in Gengma. GB30 virus strain could regularly cause illness and death in suckling mice, produced evident CPE in BHK21 cells. It agglutinated red blood cells of dove at pH5.75~7.4. This virus has been identified serologically by hemagglutination inhibition and immunofluorescent tests using Japanese encephalitis (JE), dengue (DEN) type 1,2,3,4, and chikungunya (CHIK) viruses monoclonal antibodies, and JE and sindbis (SIN) viruses immune sera. It showed specific reaction to JE virus only and no reaction with DEN 1~4, CHIK and SIN viruses. Therefore it can be identified as JE virus. This is the first report on the isolation of JE virus from Murina aurata . The results showed that bats are consider

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    Isolation of Japanese Encephalitis Virus from Brain Tissues of Bat in Yunnan Province

    • 1. Yunnan Province Institute of Epidemic Diseases Control and Research,Oali 671000,China

    Abstract: In July 1997, a strain (GB30) of virus was isolated from 60 samples of brain tissues of Murina aurata (Chiroptera: Vespertilionidae) collected in Gengma county, Yunnan province, China. Isolation of virus was negative from 4 samples of brain tissues of Rousettus leschenaulti (Chiroptera: Pteropodidae) collected in Gengma. GB30 virus strain could regularly cause illness and death in suckling mice, produced evident CPE in BHK21 cells. It agglutinated red blood cells of dove at pH5.75~7.4. This virus has been identified serologically by hemagglutination inhibition and immunofluorescent tests using Japanese encephalitis (JE), dengue (DEN) type 1,2,3,4, and chikungunya (CHIK) viruses monoclonal antibodies, and JE and sindbis (SIN) viruses immune sera. It showed specific reaction to JE virus only and no reaction with DEN 1~4, CHIK and SIN viruses. Therefore it can be identified as JE virus. This is the first report on the isolation of JE virus from Murina aurata . The results showed that bats are consider

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