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Volume 16 Issue 1
February 2001

    YE Jin, ZENG Linglan, YANG Mu, LUO Duan and GUO Jinsong. Observation of the Expression of HCV NS 5 Antigen in vitro by the SABC Immunological Techniques and Gold·labeled Colloid Electron M icroscopy Method[J]. Virologica Sinica, 2001, 16(1): 88-91.
    Citation: YE Jin, ZENG Linglan, YANG Mu, LUO Duan, GUO Jinsong. Observation of the Expression of HCV NS 5 Antigen in vitro by the SABC Immunological Techniques and Gold·labeled Colloid Electron M icroscopy Method .VIROLOGICA SINICA, 2001, 16(1) : 88-91.
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    SABC法和胶体金标免疫电镜观察 体外感染细胞中HCV—NS5的表达

    • 1.

      I同济医科大学协和医院传染科，i胡北武汉430022

    • 2.

      同济医科大学实验中心超散病理研究室，i胡北武汉430030

    • To study the expression of HCV non．structure 5 antigen in vitro，a human HepG2 cell Iine was incubated with a HCV RNA positive serum．The SABC immunologieaI techniques and gold．1abeled colloid electron microscopy method were employed tO examine for the viral proteins in those calls．The HCV non．structure 5 antigen was first detected in the HepG2 cells at 72 hours poSt ineubation．The antigen WaS continuously observed in the cytoplasm or on the membrane O．S we1I on the eell wall of the HepG2 ceils even after 1．2，3 and 4 weeks post incubation．The observation of HCV non．structure 5 antigen continuously expressed in the HepG2 cells strongly indicates that the cells may have been in． fected bv HCV virus and the virus ma y have replicated in the ce lls．Therefore．the HepG2 eell line may be served as a potentiM host for establishment of HCV infection and propagation in vitro

    Observation of the Expression of HCV NS 5 Antigen in vitro by the SABC Immunological Techniques and Gold·labeled Colloid Electron M icroscopy Method

    • 1.

      1 Department．1r lnfa-tious Disease．Xiehe Hospita1．Tongfi Medical Unlzcrsity，Wuhan 430022

    • 2.

      Lab of Ultra-mitmPathology of Experiment Ctrter．Tongji Medical University．Wuhan 430030

    • To study the expression of HCV non．structure 5 antigen in vitro，a human HepG2 cell Iine was incubated with a HCV RNA positive serum．The SABC immunologieaI techniques and gold．1abeled colloid electron microscopy method were employed tO examine for the viral proteins in those calls．The HCV non．structure 5 antigen was first detected in the HepG2 cells at 72 hours poSt ineubation．The antigen WaS continuously observed in the cytoplasm or on the membrane O．S we1I on the eell wall of the HepG2 ceils even after 1．2，3 and 4 weeks post incubation．The observation of HCV non．structure 5 antigen continuously expressed in the HepG2 cells strongly indicates that the cells may have been in． fected bv HCV virus and the virus ma y have replicated in the ce lls．Therefore．the HepG2 eell line may be served as a potentiM host for establishment of HCV infection and propagation in vitro
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        沈阳化工大学材料科学与工程学院 沈阳 110142

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      Observation of the Expression of HCV NS 5 Antigen in vitro by the SABC Immunological Techniques and Gold·labeled Colloid Electron M icroscopy Method

      • 1. 1 Department．1r lnfa-tious Disease．Xiehe Hospita1．Tongfi Medical Unlzcrsity，Wuhan 430022
      • 2. Lab of Ultra-mitmPathology of Experiment Ctrter．Tongji Medical University．Wuhan 430030

      Abstract: To study the expression of HCV non．structure 5 antigen in vitro，a human HepG2 cell Iine was incubated with a HCV RNA positive serum．The SABC immunologieaI techniques and gold．1abeled colloid electron microscopy method were employed tO examine for the viral proteins in those calls．The HCV non．structure 5 antigen was first detected in the HepG2 cells at 72 hours poSt ineubation．The antigen WaS continuously observed in the cytoplasm or on the membrane O．S we1I on the eell wall of the HepG2 ceils even after 1．2，3 and 4 weeks post incubation．The observation of HCV non．structure 5 antigen continuously expressed in the HepG2 cells strongly indicates that the cells may have been in． fected bv HCV virus and the virus ma y have replicated in the ce lls．Therefore．the HepG2 eell line may be served as a potentiM host for establishment of HCV infection and propagation in vitro

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