Enhancing Effect of Thl Cytokine on Imm une Responses of M ice to Recom binant Plasmid Encoding Hepatitis C Virus Core Gene

FENG Zhi—hua; ZHOU Yong—xing; JIAO Cheng—song; et al

PDF
Cite
Share

facebook

twitter

google

linkedin

April 2001

FENG Zhi—hua, ZHOU Yong—xing, JIAO Cheng—song, et al, Enhancing Effect of Thl Cytokine on Imm une Responses of M ice to Recom binant Plasmid Encoding Hepatitis C Virus Core Gene[J]. Virologica Sinica, 2001, 16(2): 101-104.

Citation: FENG Zhi—hua, ZHOU Yong—xing, JIAO Cheng—song, et al, . Enhancing Effect of Thl Cytokine on Imm une Responses of M ice to Recom binant Plasmid Encoding Hepatitis C Virus Core Gene .VIROLOGICA SINICA, 2001, 16(2) : 101-104.

Thl类细胞因子对pHCV．C重组体诱生免疫应答的增强作用

1.
第四军医大学唐都医院传筢科，陕西西安71003

摘要

为了探索Thl类细咆因子IL-2和IL一12对含丙型肝炎病毒(HCV)核 fl,(C)基因重组体诱生的免疫应答的增强作用．本文构建了包含HCV C基因片段的重组质粒pHCV．C，将其单独或与Thl类细胞因子表达质粒pIL一2或 pIL12共免疫BAI B／c小鼠，El ISA法检测免疫小鼠血清中的HCV C特异性抗体滴度；以pHCV—C转染SP2／0细胞．经筛选稳定表达HCV C抗原者(SP2／O HCV．C)为靶细胞． cr释放试验检测细胞毒T淋巴细胞(CTLs)的体外特异性杀伤功能。结果发现，plI，2能够增加pHCV．C诱导的抗体产生．对CTLs的杀伤作用增强却不明显：而pII ． 12对pHCV—c诱导的抗体和细胞免疫应答均有增强作用(D0 05) 由此推断，佐以Tht类细胞固子．不仅可增强基因疫苗诱生的免疫应答强度．而且可能使机体对基固疫苗的免疫应菩朝着有利于优先诱导CTLs免疫应答清除病原体的方向发展。
- 丙型肝炎病毒
- , 细胞因子
- , 基因痘苗

Enhancing Effect of Thl Cytokine on Imm une Responses of M ice to Recom binant Plasmid Encoding Hepatitis C Virus Core Gene

1.
Department Infectious Diseases，Tangdu Hospital，Fourth Military Medica[University．Xi'an 710038，China

Abstract

BALB／c mice were immunized with a recombinant plasmid containing HCV—C gene(pcD NAHCV—C)alone or together with a constructs encoding IL一2 or IL12(pIL一2 or pIL一12)．Anti— HCV—C specific antib0dy in the zer8 was measured with ELISA Spleen cells from experimental and control mice were extracted The mouse myloma SP2／0 cells expressing HCV C antigen served as tar get cells CTL activity was measured by~andard 4h Cr release ossays The results showed that anti— HCV—C antibody A value increased from 1．21 of pcDNAHCV C alone to 2 O9 and 1 73 of pHCV—C coimmunized with PIL一2 or PIL一12 respectively CTL activity to HCV core prote~n was substantially enhanced after coimmunization with the IL 12 expressing plasmid In contrast．the improvement of HCV core—specific CTL activity with an IL一2一pmdudng construct coimmunization was not obvious． whieh could related to the lOW—leve1 expression of IL 2 vector-pDOR DNA vaccine of HCV combined with IL一12 expresing plasmid co uld get better immunizing effects，and【c might represent a gene ther appeudc strategy against the HCV infection of human
- Hepatitis C virus
- , Cytokine
- , DNA vaccine

References
Proportional views

PDF

Article Metrics

Article views(3852) PDF downloads(749) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Enhancing Effect of Thl Cytokine on Imm une Responses of M ice to Recom binant Plasmid Encoding Hepatitis C Virus Core Gene

1. Department Infectious Diseases，Tangdu Hospital，Fourth Military Medica[University．Xi'an 710038，China

Abstract: BALB／c mice were immunized with a recombinant plasmid containing HCV—C gene(pcD NAHCV—C)alone or together with a constructs encoding IL一2 or IL12(pIL一2 or pIL一12)．Anti— HCV—C specific antib0dy in the zer8 was measured with ELISA Spleen cells from experimental and control mice were extracted The mouse myloma SP2／0 cells expressing HCV C antigen served as tar get cells CTL activity was measured by~andard 4h Cr release ossays The results showed that anti— HCV—C antibody A value increased from 1．21 of pcDNAHCV C alone to 2 O9 and 1 73 of pHCV—C coimmunized with PIL一2 or PIL一12 respectively CTL activity to HCV core prote~n was substantially enhanced after coimmunization with the IL 12 expressing plasmid In contrast．the improvement of HCV core—specific CTL activity with an IL一2一pmdudng construct coimmunization was not obvious． whieh could related to the lOW—leve1 expression of IL 2 vector-pDOR DNA vaccine of HCV combined with IL一12 expresing plasmid co uld get better immunizing effects，and【c might represent a gene ther appeudc strategy against the HCV infection of human