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Volume 16 Issue 2
April 2001

    SUN Cheng, CHEN Lu, ZHANG Bao, et a, Complete Nucleotide Sequennce of the S Genome Segment of Hantavirus HTN261 Strain Isolated in the Northeast China[J]. Virologica Sinica, 2001, 16(2): 140-146.
    Citation: SUN Cheng, CHEN Lu, ZHANG Bao, et a, . Complete Nucleotide Sequennce of the S Genome Segment of Hantavirus HTN261 Strain Isolated in the Northeast China .VIROLOGICA SINICA, 2001, 16(2) : 140-146.
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    汉坦病毒HTN261株s基因片段的 核苷酸序列测定及分析

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      1黑龙江省卫生防疰站病毒病防治研究所．黑龙江哈尔滨150036． 2哈尔滨兽医研究所生物技术国家重点实验室，黑龙江啥尔滨 150000

    • 黑龙江省是肾综合征出血热(HFRS)的重疫区。近年来HFRS年的发病人数曾超过万人。流行病学和血清 学研究表明黑龙江省HFRS疫区主要是姬鼠型，但目前尚缺乏病毒的分子生物学资料。我们对从瘦区捕获的宿主 动物，黑鲤姬鼠肺中分离的汉坦病毒HTN261株的S基因片段的全基因序列进行了测定和初步分析。结果如 r． HTN261株的S基因片段的全序列长为1697 nt 只有一个主要的编码N蛋白的ORF，起始位置为第37 nt，终止于1326 nt．编码的蛋白长为429 aa。没有发现存在ORlq：2。HTN261株的S基因片段核苷酸序列与HTN型中的病毒 株的劂源性很高．而与汉坦病毒其他型的同源性较差。从种系发生树分析来看．HTN261株归结于汉坦病毒的 HTN型；在HTN型之内．HTN261株和HTN76—11 8株在一个分枝内。就其核苷酸和蛋白的同源性来说． HTN261株和HTN76—118株的同源性分别是89％(全S基因)和98％(蛋白) 而与中国境内发现的其他汉坦病毒 株Z10，HId，Chert4．NC167等基因和蛋白的同源性相对较差=汉坦病毒除具有其宿主的依赖性外．还具有其地理 的簇集性。HTN261株和HTN76—118株之间S基因和N 蛋白序列的变异性的差异分别为11％ 和2％ ．表明 HTN261株和HTN?6—118株还有不同，可能是不同的亚型。不过．尚有待于进一步研究证明。

    Complete Nucleotide Sequennce of the S Genome Segment of Hantavirus HTN261 Strain Isolated in the Northeast China

    • 1.

      Institute of Viral Diseases．Provincial HealthⅡ Anti—epidemic Station of Heilongfiang，Harbin 150036、China

    • To determine the complete nuelcotide sequence of the S genome segment of HTN261 virus strain which was isolated from the striped field mouse(Apodemus agraius)in Heilongjiang province of the Northeast China．the complete nucleotide sequence of the S genome segment of HTN261 virus strain was determined with two clones，which were obtained using RT-PCR，and by linking two over lapping fragments into PUC19 vector respectively．The complete nueleotide sequence of the S genome segment of HTN261 virus strain WaS 1697 nucleotides in length and contained one ORF encoding the N protein starts at position 37 and ends at position 1326 The length of predicted protein，429 amino acids，is identieal tO the N protein of HTN，DOB，SEO types of hantaviruses Comparison of S seg— ment sequence of HTN261 strain with the corresponding sequences of other hantaviruses showed that the dentities of HTN261 strain with HTN76—118 strain were 89％ (complete nueleotide sequence) andd 98％ (amino acid sequence)．Phylogenetie ana lysis showed that HTN261 strain with HTN76— 1 18 strain form one lineage within the clade containing hantaviruses of HTN type．But the identities of HTN261 strain with HTN76—1 18 strain differ by 1 1％ in complete nucleotide sequence of S segment and 2％ in amino acid sequence of N protein．HTN261 strain belongs to hantavirus HTN type and may be a new subtype
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      Complete Nucleotide Sequennce of the S Genome Segment of Hantavirus HTN261 Strain Isolated in the Northeast China

      • 1. Institute of Viral Diseases．Provincial HealthⅡ Anti—epidemic Station of Heilongfiang，Harbin 150036、China

      Abstract: To determine the complete nuelcotide sequence of the S genome segment of HTN261 virus strain which was isolated from the striped field mouse(Apodemus agraius)in Heilongjiang province of the Northeast China．the complete nucleotide sequence of the S genome segment of HTN261 virus strain was determined with two clones，which were obtained using RT-PCR，and by linking two over lapping fragments into PUC19 vector respectively．The complete nueleotide sequence of the S genome segment of HTN261 virus strain WaS 1697 nucleotides in length and contained one ORF encoding the N protein starts at position 37 and ends at position 1326 The length of predicted protein，429 amino acids，is identieal tO the N protein of HTN，DOB，SEO types of hantaviruses Comparison of S seg— ment sequence of HTN261 strain with the corresponding sequences of other hantaviruses showed that the dentities of HTN261 strain with HTN76—118 strain were 89％ (complete nueleotide sequence) andd 98％ (amino acid sequence)．Phylogenetie ana lysis showed that HTN261 strain with HTN76— 1 18 strain form one lineage within the clade containing hantaviruses of HTN type．But the identities of HTN261 strain with HTN76—1 18 strain differ by 1 1％ in complete nucleotide sequence of S segment and 2％ in amino acid sequence of N protein．HTN261 strain belongs to hantavirus HTN type and may be a new subtype

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