本文报道了口蹄疫病毒(F∞t—and-Mouth Disease Vh’us FMDV)在体外诱导PK．15细胞消亡的研究结果。采 用Hoechst33258荧光探针、DNA凝胶电泳、脱氧核搪核酸转移酶舟导的缺口末端标记(TUNEL)技术均检测到了 典型的细胞凋亡 结果显示：使用感染性滴度为4 8lgTCI ／mL的口蹄疫病毒感染PK一15细胞，在培养32 h后r 荧光探针检测呈现典型的凋亡细胞核固缩和梅花状碎裂核，并伴随有凋亡小体出现，凋亡率约为20％ ；DNA凝胶 电诛显示ladder梯带；末端标记检测到强绿色荧光标记物结合于凋亡细胞核上 研究结果提示：口蹄疫病毒可以 在体外诱导宿主细胞隅亡，细胞偶亡是其致细胞病交死亡的重要途径之一。

Apoptosis of PK—t5 ceils induced by Foot—and-Mouth Disease Virus(FMDV)in vitro was reported in this paper．Typical cell apoptosis was detected hy ti．se of Hoechst 33258 fluorescence probe． agarose gel electrophoresis and in situ end-labeling(TUNEL)．After PK一15 cells were infected by titration of 4．8 lg TCIDsD／mL FMDV for 32 h，apoptosis characteristics of nuclear condensation， fragmentation，accompanied by apoptotic bodies formation(Hoeehst 33258 staining)．180—200 inte— ger-fold sized pieces DNA Ladders(agarose gel e1．ectrophoresis)and strong green fluorescence clots (TUNEL)were alI exhibited，and cell apoptosis was approximately 20％ In addition．the quantira— tire analysis of apoptosis in PK一15 ceils induced by FMDV showed that apoptosis was correlated with infection of virus．and it was also tim~dependent．Results indicare that FMDV can induce apoptosis of host ceils and apoptosis plays an important role in the cytopathogencity effect of FMDV