REN Li—hong.BAI W ei, LIN Ling, SUN Ming, LI Hua, YI Hong—kun, HOU Zhong—liu, Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood[J]. Virologica Sinica, 2001, 16(2): 179-182.
Citation: REN Li—hong.BAI W ei, LIN Ling, SUN Ming, LI Hua, YI Hong—kun, HOU Zhong—liu, . Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood .VIROLOGICA SINICA, 2001, 16(2) : 179-182.

猴血中SFV病毒基因PCR检测方法的建立及应用

  • 针对猴泡沫病毒SFV(Simian Foamy Viras)多聚酶区(pd区)设计两对引物,以猴血DNA为模顿进行嵌套式 PCR扩增 得到500 bp左右基因片段,克隆进入pUC一19载体,经测序鉴定为SFV 465 bp的po】区基因片段 将此 段序列与SFV各型425 bp的p 区基因片段进行阿源性比较,它与SFV-1型的同源性最高,为92.O0%。在此基础 上,用这两对引物对158例猴血DNA进行检测,阳性54例,阳性率为34 2%。发现在猴群中有较高的SFV病毒 的感染。

Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood

  • Two pairs of primers were used for amplifying SFV gene with nested—PCR in Rhesus Mon— key blood The neady 500 bp PCR products were cloned into vector pUC-19.The fragment was de tected as 465 bp SFV pol gene by sequence analysis.The homology of the fragment to 425 bp pol gene of the SFV-1 was the highest.it was 92 00% 158 samples of Rhesus Monkey blood were used to de teet SFV gene with nested—PCR Among them,54 were positive.the poskive rate was 34 2% h ap— peared a h/gh present of SFV infection in Rhesus M onkey.

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    Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood

    • 1. Institute of Medical Biology,CAMS & PUMC.Kunming 650118.China

    Abstract: Two pairs of primers were used for amplifying SFV gene with nested—PCR in Rhesus Mon— key blood The neady 500 bp PCR products were cloned into vector pUC-19.The fragment was de tected as 465 bp SFV pol gene by sequence analysis.The homology of the fragment to 425 bp pol gene of the SFV-1 was the highest.it was 92 00% 158 samples of Rhesus Monkey blood were used to de teet SFV gene with nested—PCR Among them,54 were positive.the poskive rate was 34 2% h ap— peared a h/gh present of SFV infection in Rhesus M onkey.

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