U Lan, CHEN Xin-wen, SUN Xiulian, Hilary Lallzon, Just M, Vlak Basil and HU Zhi-hong. Sequence Analysis of the Putative-cathepsin Gene of Heliothis Armigera Single-nudeocapsid Baculovirus[J]. Virologica Sinica, 2001, 16(3): 229-235.
Citation:
U Lan, CHEN Xin-wen, SUN Xiulian, Hilary Lallzon, Just M, Vlak Basil, HU Zhi-hong.
Sequence Analysis of the Putative-cathepsin Gene of Heliothis
Armigera Single-nudeocapsid Baculovirus .VIROLOGICA SINICA, 2001, 16(3)
: 229-235.
棉铃虫单核衣壳核多角体病毒组织蛋白酶基因的
序列分析和进化研究
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摘要
对棉铃虫单核衣壳核多角体病毒 (HaSNPV)的组织蛋白酶 ( cath)基因进行了序列分析 ,此基因编码区长 10 98bp ,预计编码一个 36 6个氨基酸的蛋白产物。序列分析表明 ,HaSNPV的V CATH蛋白与其它杆状病毒的同源蛋白具有相似的保守结构并保留有相同的酶活性位点 ,根据已知的杆状病毒组织蛋白酶序列构建了 cath基因的进化树 ,发现HaSNPV的 cath位于NPV组中一个单独的分枝 ,结合 cath基因在棉铃虫病毒基因组中的位置与其它NPV有较大不同 ,推测HaSNPV的 cath基因可能拥有特殊的进化历程。
Sequence Analysis of the Putative-cathepsin Gene of Heliothis
Armigera Single-nudeocapsid Baculovirus
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Abstract
A putative -cathespin(cath)gene was identified from the single(S) nucleocapsid nucleopolyhedrovirus of Helicoverpa armigera(HaSNPY).This open reading frame is 1098 nucleotides long,encoding a putative protein of 366 amino acids.The amino acids sequence alignment of eleven baculoviral v-CATHs showed that the primary structure and the catalytic sited of the proteins were conserved.A pholygenetic tree of these v-CATHs was constructed by using maximun parsimony analysis,the result indicated that the -cath gene of HaSNPV had a different evolutionary history from that of other NPVs.
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