WU Jia-cai and ZHANG Chuan-xi. Sequence Analysis of the Helicoverpa armigera Nuclear Polyhedrosis Virusp40 Gene[J]. Virologica Sinica, 2001, 16(3): 242-245.
Citation: WU Jia-cai, ZHANG Chuan-xi. Sequence Analysis of the Helicoverpa armigera Nuclear Polyhedrosis Virusp40 Gene .VIROLOGICA SINICA, 2001, 16(3) : 242-245.

棉铃虫核型多角体病毒p40基因序列分析

  • 克隆了棉铃虫(He//an~rpa帅面 %)单粒包埋型核型多角体病毒(n iPv)基因组胁 m.H、J片段,其长度分 别为10 kb和6 7kb。通过对克隆片段末端测序,得到HaSNPV p40基因全序列。p40基因编码区全长966bp,预计可 编码36.kn的多肽。n iPv p40基因核苷酸序列与flzSNIW p40基因有98%的相同性。这两种基因与BmNP p40 (&ⅡBa|lk—L331~0)和Ael~IPV~41(C,enBank—L22858)的氨基酸序列相似性43%左右,但四种蛋白对应于H~NPV p4o、HaSNPV v4o的28 277氨基酸区域,同源性高达62%,并且存在一个保守的亲水性结构域。进一步将在基因 组中相邻的胁tdⅢ 一H、J两片段用BamH I、 RI、Hind m、Psti四种限制性内切酶进行了分析。

Sequence Analysis of the Helicoverpa armigera Nuclear Polyhedrosis Virusp40 Gene

  • The Hind Ⅲ-H?J fragments of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) qenome were cloned. By sequencing the terminus of the cloned fragments, an ORF encoding the HaSNPV p40 was identified. The coding region was 966 bp long and potential to encode a 36.6kD pepetide. The HaSNPV p40 exhibits 98% identity with HzSNPV p40 at the nucleotide sequence. The two genes diverged significantly from BmNPV p40 and AcMNPV gp41.The predicted amino acid sequences have less than 45% homology between HaSNPV p40?HzSNPV p40 and BmNPV p40 or AcMNPV gp41, but four genes have a conserved hydrophilic domain. In this region they have 62% homology. Further more, we determined that Hind Ⅲ-H fragment links with J fragment in the genome and the two fragments were analasysed with four restriction endonucleases (BamH Ⅰ?EcoR Ⅰ?Hind Ⅲ?Pst Ⅰ) .

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    Sequence Analysis of the Helicoverpa armigera Nuclear Polyhedrosis Virusp40 Gene

    • 1. Institute of Applied Entomology,Zhejiang University,Hangzhou 310029,china

    Abstract: The Hind Ⅲ-H?J fragments of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) qenome were cloned. By sequencing the terminus of the cloned fragments, an ORF encoding the HaSNPV p40 was identified. The coding region was 966 bp long and potential to encode a 36.6kD pepetide. The HaSNPV p40 exhibits 98% identity with HzSNPV p40 at the nucleotide sequence. The two genes diverged significantly from BmNPV p40 and AcMNPV gp41.The predicted amino acid sequences have less than 45% homology between HaSNPV p40?HzSNPV p40 and BmNPV p40 or AcMNPV gp41, but four genes have a conserved hydrophilic domain. In this region they have 62% homology. Further more, we determined that Hind Ⅲ-H fragment links with J fragment in the genome and the two fragments were analasysed with four restriction endonucleases (BamH Ⅰ?EcoR Ⅰ?Hind Ⅲ?Pst Ⅰ) .

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