ZOU Jian, SHEN Xuc-ten, WU Jhn·hua, ZHOU Guo·ying and GONG Zu·xllnl“. Construction of the Transgenic Plasmid Containing GFP Gene and Four Ribozyme Genes Targeting the Prawn Baclll0virus Gene[J]. Virologica Sinica, 2001, 16(3): 275-279.
Citation: ZOU Jian, SHEN Xuc-ten, WU Jhn·hua, ZHOU Guo·ying, GONG Zu·xllnl“. Construction of the Transgenic Plasmid Containing GFP Gene and Four Ribozyme Genes Targeting the Prawn Baclll0virus Gene .VIROLOGICA SINICA, 2001, 16(3) : 275-279.

含四个串联核酶和GFP基因的转基因质粒的构建

  • 以含绿色荧光蛋白(crP)基因的质粒pSK100-DS、含切害j对虾杆状病毒基因的核酶P,zl的质粒pRG Rzl、含核 酶Rz2的质粒pRG Rr2和转基因空质粒peDNA3为基础.把绿色荧光蛋白GFP基因克隆于[,eDNA3的SV40启动了 下面,由SV40启动子控制:含四个两种核酸基因的四联体克隆于pd~A3的多克隆位点区,由T7启动子控制.构建 成含两个Rzl、两个Rr2和GFP基因的转基因质粒pGTR,以用于转基因抗病毒对虾的研究。

Construction of the Transgenic Plasmid Containing GFP Gene and Four Ribozyme Genes Targeting the Prawn Baclll0virus Gene

  • TheⅡ lsg c plasⅡl|d of haⅡⅡI1e r}Iead ribozymes Rzl and Rz2 targetirg one of the Prat,m bac· ulovims genes has been eoustmctae.The transgenic plasmld eontains double series o0pjes of Rzl and Rz2 as Rzl·Rz2·Rzl·Rz2.The Green Fluorescent Protein(GFP)gene was selected a8 the report gene of the transgenic plasmid.The GFP gene was cloned into plasmld peDNA3 at site 2093bp with Sma I.cont~lled by SV40 pro· illlOter.mb0 Ⅱ】e gene Seile8wclonedintotheMCS ofplasmld peDNA3with proper rest6cfion endonuclease, conmdled byT7 andCMV promoter.The constructedtransgenlc plasmidw∞ named andwill be usedfor obtainingta’ansgenic Prawn.

  • 加载中
  • 加载中

Article Metrics

Article views(3860) PDF downloads(786) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Construction of the Transgenic Plasmid Containing GFP Gene and Four Ribozyme Genes Targeting the Prawn Baclll0virus Gene

    • 1. Institute of Biochemistry and cell biology,Shanghai Institute for biological Sciences,Chinese academy of sciences,shanghai 200031,china

    Abstract: TheⅡ lsg c plasⅡl|d of haⅡⅡI1e r}Iead ribozymes Rzl and Rz2 targetirg one of the Prat,m bac· ulovims genes has been eoustmctae.The transgenic plasmld eontains double series o0pjes of Rzl and Rz2 as Rzl·Rz2·Rzl·Rz2.The Green Fluorescent Protein(GFP)gene was selected a8 the report gene of the transgenic plasmid.The GFP gene was cloned into plasmld peDNA3 at site 2093bp with Sma I.cont~lled by SV40 pro· illlOter.mb0 Ⅱ】e gene Seile8wclonedintotheMCS ofplasmld peDNA3with proper rest6cfion endonuclease, conmdled byT7 andCMV promoter.The constructedtransgenlc plasmidw∞ named andwill be usedfor obtainingta’ansgenic Prawn.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return