为研究汉坦病毒1(24株M和S片段基因的结构特征，从病毒感染细胞提取细胞总RNA，经 个循环的一次 性PCR得到了与理论值相符合产物，将RT—PCR产物直接克隆T载体，经K24毒株M片段的全基因序列共3 653 个核苷酸，四种核苷酸的酶切和PCR鉴定正确的克隆通过柱纯化后进行序列测定，结果比例分别为A 30．44％ ，T 30．14％，G 20．72％，C18．70％ ，GC含量为39．42％ ．AT含量为6o 58％，编码1 133个氪基酸。S片段的全基因序列 共1 772个核苷酸，四种核苷酸的比例分别为A．31 30％，G26．05％，"1’22．79％ ，C19．77％，GC含量为45．81％ ，AT含量 为54 19％，共编码429个氨基酸。1(24株M片段桉苷酸全基因和氪基酸与HTN型毒株同源率分别为70．7％ 一 71．2％和75．9％ 一76．9％ ，与SEO型为95 2％ ～99．2％和95 9％ 一99．4％。S片段与I／rlq 76 118和SEO SR-II病 毒的比较，核苷酸同源率分别为74．0％和95 6％，氨基酸同源率为83．3％和97．9％ ，与M片段的结果相类似。M 片段氪基酸与SEO型1O个毒株存在4个氪基酸替代。应用DNASTAR软件的系统发生分析方法分别绘出了基于M 片段核苷酸厦推导氪基酸的系统发生树。

Abstract：Our pmvious studv showedthatK24 strain懈arI antigenicity broadHantavims SE0 type strain．In this paper we studied the molecular characteristics of K24 strain．The total ceUular RNA Wilts extracted from in． fected cell culture andtheM and S segment genewere ampficated byRT-PCR andthen cloned and sequenced． The resultsindlcated thattheK24 strain completeM genome segment w丑s 3 652 nucleotidein length encoding 1 133amino acid，andthe complete S gmaon~segmoatwas1 772 nucleotideeneoa~ 429mnino acid．C0Ⅱ ． ingwith other hmatavirus rIs。the homology． of M genmne segment with that of H] ,Arms is 70．7％ 一 71．2％ in nucleotide leveland75．9％ 一76．9％ in amino acid level respectively．wh wit}l that ofSEO virus strains it WaS 95．2％ 一99．2％ at nucleotide level and 95．9—99．4％ at amino acidleve1．The homology ofS gen ome segment with H] 76．118 and SEO SR一1l showed 74．O％ and 95．6％ at nucleotide leve1．and 83． 3％ and 97．9％ at amino acidlevel_Thetesul：s a similartothat ofM gt~qome segment．Wh印c哪I with 10 SEO virus stu ns，therewere 4 amino acid diferencesintheM gencnae segment，whcihmi t be 叩m1sible an tigeniclty characteristics broad．Phylogenictrees We constructed depending OI1the es ofril1． cleotide and mnino acids respectively．