CU Wen-jie.LIN Jing-xiang, WANC You-chun“, ZHANC.I~a-yuan, LAN Hai.yun, LI He-rai and The Sequence Analysts of I-IB~ S Gene Isolated from Both m 3sAgand Anti-l-IBs Antibody Positive Blood Donors[J]. Virologica Sinica, 2001, 16(4): 321-324.
Citation: CU Wen-jie.LIN Jing-xiang, WANC You-chun“, ZHANC.I~a-yuan, LAN Hai.yun, LI He-rai, . The Sequence Analysts of I-IB~ S Gene Isolated from Both m 3sAgand Anti-l-IBs Antibody Positive Blood Donors .VIROLOGICA SINICA, 2001, 16(4) : 321-324.

乙肝病毒表面抗原和抗体双阳性者中病毒S区基因序列分析

  • 为了解乙肝病毒 (HBV)表面抗原和抗体双阳性患者中病毒的基因型及其HVBS区是否有变异。用放射免疫试剂检测HBsAg阳性样品中的抗 HBs抗体 ,用聚合酶链反应法检测双阳性样品中的HBVDNA ,然后对阳性样品进行克隆和基因序列分析 ,并将所得序列与HBV不同基因型的代表株进行比较分析。结果显示 389例HBsAg阳性样品中有 10例为抗HBs抗体阳性 ;该 10例双阳性样品中有 5例为HBVDNA阳性 ;序列分析显示该 5株HBV均为B基因型 ,其中 4株为adw亚型 ,1株为adr亚型 ;其中有 2株在S区的“a”决定簇的氨基酸发生了变异In order to investigate HBV genotype and variants from the blood donors positive for both HbsAg and anti HBs antibody,389 sera from HBsAg positive blood donors were tested for anti HBs antibody with RIA assay.The sera positive for both HbsAg and anti HBs were tested for HBV DNA with polymerase chain reaction(PCR).The positive PCR products were cloned and sequenced.The sequences were compared with the representative sequences of different HBV genotypes.The results showed that 10/389 sera positive for HBsAg were positive for anti HBs.Five of these ten sera were positive for HBV DNA.The sequence analysis showed that all five sequences belonged to HBV genotype B,four sequences were adw serotype and one was adr serotype.Amino acids of antigen determinant "a" of two sequences have been changed.

The Sequence Analysts of I-IB~ S Gene Isolated from Both m 3sAgand Anti-l-IBs Antibody Positive Blood Donors

  • In order to investigate HBV genotype and variants from the blood donors positive for both HbsAg and anti HBs antibody,389 sera from HBsAg positive blood donors were tested for anti HBs antibody with RIA assay.The sera positive for both HbsAg and anti HBs were tested for HBV DNA with polymerase chain reaction(PCR).The positive PCR products were cloned and sequenced.The sequences were compared with the representative sequences of different HBV genotypes.The results showed that 10/389 sera positive for HBsAg were positive for anti HBs.Five of these ten sera were positive for HBV DNA.The sequence analysis showed that all five sequences belonged to HBV genotype B,four sequences were adw serotype and one was adr serotype.Amino acids of antigen determinant "a" of two sequences have been changed.

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    The Sequence Analysts of I-IB~ S Gene Isolated from Both m 3sAgand Anti-l-IBs Antibody Positive Blood Donors

    • 1. National Institute for the Control of Parmeceutical and Biological Products,Beijing 100050,China

    Abstract: In order to investigate HBV genotype and variants from the blood donors positive for both HbsAg and anti HBs antibody,389 sera from HBsAg positive blood donors were tested for anti HBs antibody with RIA assay.The sera positive for both HbsAg and anti HBs were tested for HBV DNA with polymerase chain reaction(PCR).The positive PCR products were cloned and sequenced.The sequences were compared with the representative sequences of different HBV genotypes.The results showed that 10/389 sera positive for HBsAg were positive for anti HBs.Five of these ten sera were positive for HBV DNA.The sequence analysis showed that all five sequences belonged to HBV genotype B,four sequences were adw serotype and one was adr serotype.Amino acids of antigen determinant "a" of two sequences have been changed.

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