Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm

ZHANG Xiao-xia．ZHANG Zhong-xin．DING Qing-quan

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August 2001

ZHANG Xiao-xia．ZHANG Zhong-xin．DING Qing-quan. Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm[J]. Virologica Sinica, 2001, 16(4): 338-343.

Citation: ZHANG Xiao-xia．ZHANG Zhong-xin．DING Qing-quan. Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm .VIROLOGICA SINICA, 2001, 16(4) : 338-343.

棉铃虫核多角体病毒iap2基因的克隆和序列分析

张小霞张忠信丁清泉

1.
中国科学院武汉病毒研究所，湖北武汉430071

摘要

棉铃虫核多角体病毒 (Helicoverpaarmigerasinle nucleocapsidnucleopolyhedrovirus,HaSNPV)基因组中发现了一个抗细胞凋亡基因iap2。该基因位于病毒基因组的BamHI F片段 ,全长 75 3个核苷酸 ,编码 2 5 0个氨基酸 ,预计蛋白质分子量 2 9.2kD。在iap2基因上游 - 30～ - 33的位置有一个TATA框 ,在 5 0～ 5 3的位置有一个早期转录信号CAAT ,在终止密码子下游有一个poly(A)信号AATAAA ,HaSNPViap2基因编码一个锌指结构和两个BIR结构。与LdMNPV、AcMNPV、SeMNPV和OpMNPV的IAPs及果蝇DIAP2和人类XIAP等九种IAPs相比较 ,HaSNPVIAP2与其它的BIR(baculovirusIAPrepeater)和RING结构在Cys/His基元 (motif)位点上高度保守 An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A"TATA"box was found-30～-33nt upstream of the translational start codon ATG.There is an early consensus translational motif CAAT,-50～-53nt upstream of the translational start codon ATG.A typical poly(A)signal was identified 46～51nt downstream of the translational stop codon TAA.HaSNPV iap 2 gene encode one typical RING and two BIRs.The alignment of AcMNPV?OpMNPV?LdMNPV?LdMNPV?SeMNPV?DIAP and XIAP indicated that they had consensus BIR motifs and RING zinc finger motif.It was suggested HaNPV IAP2 had a potential anti apoptotic function.
- 棉铃虫核多角体病毒
- , 细胞凋亡
- , BIRs
- , 锌指结构
- , IAP
- , Helicoverpa
- , armigera
- , single
- , nucleocapsid
- , nucleohedrovirus
- , IAP
- , RING
- , BIR
- , Homologue

Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm

ZHANG Xiao-xia．ZHANG Zhong-xin．DING Qing-quan

1.
Wuhan institute of Virology, Academy of Sicences，Wuhan 430071,china

Abstract

An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A"TATA"box was found-30～-33nt upstream of the translational start codon ATG.There is an early consensus translational motif CAAT,-50～-53nt upstream of the translational start codon ATG.A typical poly(A)signal was identified 46～51nt downstream of the translational stop codon TAA.HaSNPV iap 2 gene encode one typical RING and two BIRs.The alignment of AcMNPV?OpMNPV?LdMNPV?LdMNPV?SeMNPV?DIAP and XIAP indicated that they had consensus BIR motifs and RING zinc finger motif.It was suggested HaNPV IAP2 had a potential anti apoptotic function.
- Helicoverpa armigera single nucleocapsid nucleohedrovirus IAP RING BIR Homologue

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nudeocapsid Nudeopolyhedrovinm

ZHANG Xiao-xia．ZHANG Zhong-xin．DING Qing-quan

1. Wuhan institute of Virology, Academy of Sicences，Wuhan 430071,china

Abstract: An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A"TATA"box was found-30～-33nt upstream of the translational start codon ATG.There is an early consensus translational motif CAAT,-50～-53nt upstream of the translational start codon ATG.A typical poly(A)signal was identified 46～51nt downstream of the translational stop codon TAA.HaSNPV iap 2 gene encode one typical RING and two BIRs.The alignment of AcMNPV?OpMNPV?LdMNPV?LdMNPV?SeMNPV?DIAP and XIAP indicated that they had consensus BIR motifs and RING zinc finger motif.It was suggested HaNPV IAP2 had a potential anti apoptotic function.