FANG Ming-gang, CHEN Xin, WANG IJuan-zhong, Just M and HU Zhi-hong. Sequence Analysis of the H/rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV)[J]. Virologica Sinica, 2001, 16(4): 355-360.
Citation: FANG Ming-gang, CHEN Xin, WANG IJuan-zhong, Just M, HU Zhi-hong. Sequence Analysis of the H/rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV) .VIROLOGICA SINICA, 2001, 16(4) : 355-360.

棉铃虫单核衣壳核多角体病毒(HaSNPV)Hind III.L片段的序列分析

  • 本文报道了棉铃虫单核衣壳核多角体病毒 (Helicoverpaarmigerasingle nucleocapsidnucleopolyhedrovirus,HaSNPV)基因组的HindIII L片段的全序列。该片段全长 2 6 35bp ,包括 5个有意义的开放阅读框 :HaSNPVORF2 2 7,晚期表达因子 10基因 (lef10 ) ,vp10 5 4基因 ,Ac5 5 (AcMNPVORF5 5的同源基因 ) ,Ac5 6 (AcMNPVORF5 6的同源基因 )。与其它 6种杆状病毒的氨基酸序列比较表明 ,HaSNPV的lef10基因与甜菜夜蛾核型多角体病毒 (SeMNPV)的同源性最高 ,为6 4 % ,与冷杉毒蛾核型多角体病毒 (OpMNPV)的同源性最低 ,为 4 3% ;HaSNPV的vp10 5 4基因与SeMNPV的同源性最高 ,为 6 5 % ,与OpMNPV的同源性最低 ,为 4 9%。序列比较表明 ,HaSNPV的LEF10与VP10 5 4蛋白与其它 6种杆状病毒具有相同的保守区和亮氨酸拉链 (leucinezipper)In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a late expression factor 10 gene ( lef 10),a baculovirus structural protein gene( vp 1 054), Ac 55(a homologue of the AcMNPV ORF55) and Ac 56 (a homologue of the AcMNPV ORF56),respectively.These five genes were identified for the first time in HaSNPV.Alignment of baculovirus LEF10 s indicates that they have conserved leucine zipper regions,and aligament of baculovirus VP1054s indicates that they are highly conserved.

Sequence Analysis of the H/rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV)

  • In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a late expression factor 10 gene ( lef 10),a baculovirus structural protein gene( vp 1 054), Ac 55(a homologue of the AcMNPV ORF55) and Ac 56 (a homologue of the AcMNPV ORF56),respectively.These five genes were identified for the first time in HaSNPV.Alignment of baculovirus LEF10 s indicates that they have conserved leucine zipper regions,and aligament of baculovirus VP1054s indicates that they are highly conserved.

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    Sequence Analysis of the H/rid m -L Region of the Helicoverpa armigera Single-nudeocapsid Nucleopolyhedrovirus(HaSNPV)

    • 1. 中国科学院武汉病研究所无脊椎动物病毒学联合开放实验室 湖北武汉 Lab of Virology Laboratory Wageningen University Bennenhaven11 6709

    Abstract: In order to investigate the genetic organization of the single nucleocapsid nucleopolyhedrovirus (SNPV) of Helicoverpa armigera (HaSNPV),the Hin d III L fragment of the viral genome was sequenced.The fragment contained five complete open reading frames(ORFs) representing ORF227 (a unique gene),a late expression factor 10 gene ( lef 10),a baculovirus structural protein gene( vp 1 054), Ac 55(a homologue of the AcMNPV ORF55) and Ac 56 (a homologue of the AcMNPV ORF56),respectively.These five genes were identified for the first time in HaSNPV.Alignment of baculovirus LEF10 s indicates that they have conserved leucine zipper regions,and aligament of baculovirus VP1054s indicates that they are highly conserved.

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