CHANG Guo-hui.CHEN Sheng—hang“, LUO Ban-jun, u Tian-xia and Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus[J]. Virologica Sinica, 2001, 16(4): 390-392.
Citation: CHANG Guo-hui.CHEN Sheng—hang“, LUO Ban-jun, u Tian-xia, . Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus .VIROLOGICA SINICA, 2001, 16(4) : 390-392.

茶毛虫核型多角体病毒【EpNPV】多角体蛋白基因的定位及克隆

  • 茶毛虫核型多角体病毒 (Euproctispseudoconsper saNuclearPolyhedrosisVirus简称EpMPV) ,属于杆状病毒科核型多角体病毒属 ,能使茶毛虫染病死亡。EpNPV据报道最初由日本学者于 195 7年发现[1] ,随后在其它国... The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was cloned into pTZ19R vector.Then the fragment was digested by Xba I? Hin d III,and subcloned into pTZ19R.

Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus

  • The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was cloned into pTZ19R vector.Then the fragment was digested by Xba I? Hin d III,and subcloned into pTZ19R.

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    Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus

    • 1. 慨血mlnsdace o厂V/rdogy,Ch/r~e缸I sc~nce*,Wuhan 430071.0

    Abstract: The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was cloned into pTZ19R vector.Then the fragment was digested by Xba I? Hin d III,and subcloned into pTZ19R.

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