Cloning and Sequence Analysis of Chinese CXCR4

FAN Zhao; I I; LUO gao-jun

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Volume 17 Issue 1

February 2002

FAN Zhao, I I, LUO gao-jun and Cloning and Sequence Analysis of Chinese CXCR4[J]. Virologica Sinica, 2002, 17(1): 6-10.

Citation: FAN Zhao, I I, LUO gao-jun, . Cloning and Sequence Analysis of Chinese CXCR4 .VIROLOGICA SINICA, 2002, 17(1) : 6-10.

中国人HIV辅助受体CXCR4基因的克隆及序列测定

1.
1.中国科学院武汉病毒研究所，湖北武汉430071
2.
军事医学科学院微生物流行痛研究所，北京100071

摘要

摘要：为了获得HIv辅助受俸CXCR4基因，进一步探索艾滋扁(AlD6)的诊断、防治方法，采用逆转录巢式聚合酶链式反应(RT-nPCR)从中国人外周血淋巴细胞中克隆了CXCR4 cDNA基因．得到了预期的1 100bp左右的片段。将PCR产物与T载俸连接．并进行了酶切反应鉴定，获得与T载俸正向连接的克隆。测序结果表明：克隆的片段古有一十开放性阅读框架．编码352个氨基酸残基。搜索GenBank．目标片段与M99293、XM051223、XM051224、 XM051225有很高的同源性，比较结果显示：克隆的片段包括完整的编码序列．舍两十碱基的差异。
- CXCR4
- , cDNA克隆：序列分析

Cloning and Sequence Analysis of Chinese CXCR4

1.
1 Wuhan Instituteof virology，Chinese Academy of Sciences．Wuhan 430071．China
2.
Institute of Microbiology and Epidemology，Academy of Military．Medical Sciences．Beijing 100071，China

Abstract

Abstrach In order to acquire the CXCR4 gene and develop the method to diagnose and treat AIDS pa— tient，the cDNA of CXCR4 was doned by RT-nPCR from peripheral blood mononuclear cells of a Chi— nese．An expected target fragment was identified by restriction enzyme digestion and DNA sequence data．The sequence data showed that CXCR4 gene is 1 059 nt encoding 352 amino acids．Compared with the sequence from GeneBank，there were only two deferent sites．Searched in GenBank，it was found that the segment had high homologenicity with M99293、XM 051223、XM051224、XM05 1225． The comparison result showed that the cloned segment included intact cod ing sequence and two base． pair deference
- CXCR4：cDNA cloning
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequence Analysis of Chinese CXCR4

1. 1 Wuhan Instituteof virology，Chinese Academy of Sciences．Wuhan 430071．China
2. Institute of Microbiology and Epidemology，Academy of Military．Medical Sciences．Beijing 100071，China

Abstract: Abstrach In order to acquire the CXCR4 gene and develop the method to diagnose and treat AIDS pa— tient，the cDNA of CXCR4 was doned by RT-nPCR from peripheral blood mononuclear cells of a Chi— nese．An expected target fragment was identified by restriction enzyme digestion and DNA sequence data．The sequence data showed that CXCR4 gene is 1 059 nt encoding 352 amino acids．Compared with the sequence from GeneBank，there were only two deferent sites．Searched in GenBank，it was found that the segment had high homologenicity with M99293、XM 051223、XM051224、XM05 1225． The comparison result showed that the cloned segment included intact cod ing sequence and two base． pair deference