HOU Wei‘, YANG Zhan-qiu‘”, Qu Hong .ZHAO Ming, XIAO Hong, CAI H 一lan and Study on M Genomic Segment of Hantaviros Strains Isolated from Different Areas in Hubei Province[J]. Virologica Sinica, 2002, 17(1): 26-29.
Citation: HOU Wei‘, YANG Zhan-qiu‘”, Qu Hong .ZHAO Ming, XIAO Hong, CAI H 一lan, . Study on M Genomic Segment of Hantaviros Strains Isolated from Different Areas in Hubei Province .VIROLOGICA SINICA, 2002, 17(1) : 26-29.

湖北省不同地区汉坦病毒M片段的分析研究

  • 参考汉坦病毒(Hv)的基因文库软件设计M 片段G1区型特异性引物, I-IV 76/118、H一114、A9及R2 株 RNA为阳性模板,建立HV的分型方法——逆转录一半巢式聚合酶链匣应(RT-heminested PCR),对湖北省不同地 区分离的30株HV进行分型。结果显示:(1)建立的RT-heminested PCR分型方法对I-IV两型的代表株76/118 (HTN)、A9(HTN)、H一114(HTN)及 2(sE0)RNA进行丁特异性扩增,其大小与理论值一致;此法只能检测HV RNA,不能检测其它病毒RNA。(2)分离于湖北省不同地区的30株Hv的分型结果为汉滩型21株、授城型9株, 其中长江 南汉滩型12株、汉城型2株;长江以北汉濉型9株、汉城型7株。这表明建立的RT—heminested PCR用 于HV的检测特异性好;分析湖北省不同地区分离的30株HV,显示湖北省HFRS流行为混合疫区;且在湖北地区 具有一定的地理聚集性。

Study on M Genomic Segment of Hantaviros Strains Isolated from Different Areas in Hubei Province

  • Abstract:According tO Genebank sofeware of HV.the type-specific primers were desisgned cori-~s— pording tO the conserved sequences in the GI region of M genomic segment of HV.A reverse—transcrip— tion heminested polymerase chain reaction for HV was developed.Then.RT-heminested PCR was ap— plied tO type 3O strains of HV isolated from different areas in Hube i province.The results are shown that:(1)The 76/118、A9、H-114(HTNV)and R22(SEOV)Strains of HV were amplified and typed using the RT-heminested PCR.And HV RNA could only be detected by this method.but other vira1 RNA couldn’t be detected .(2)30 strains isolated from different areas in Hubei province were typed as HTNV amd SEOV。respectively.Those results suggested that:(1)The RT-heminested PCR was lea— sible for detectiong and typing HV in Hubei province.(2)Both HINV and SEOV were found in eoid— mic aro-.~.S of HFRS in Hubei province.Distinct hantaviruses could coexist in either different or the sam e geographic or ecological zones in Hubei province.

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    Study on M Genomic Segment of Hantaviros Strains Isolated from Different Areas in Hubei Province

    • 1. 1.Institute of Virology.Medical CollegeofWuhan University,Wuhan 430071,China
    • 2. Department ofMicrobiology,Ton MedicalCollegeof Hva~ g Uiversityof Science and Technology.Wuhan 430030.China
    • 3. Departmentof Infection,the ThirdHospital Wuhan,Wuhan 430060.China

    Abstract: Abstract:According tO Genebank sofeware of HV.the type-specific primers were desisgned cori-~s— pording tO the conserved sequences in the GI region of M genomic segment of HV.A reverse—transcrip— tion heminested polymerase chain reaction for HV was developed.Then.RT-heminested PCR was ap— plied tO type 3O strains of HV isolated from different areas in Hube i province.The results are shown that:(1)The 76/118、A9、H-114(HTNV)and R22(SEOV)Strains of HV were amplified and typed using the RT-heminested PCR.And HV RNA could only be detected by this method.but other vira1 RNA couldn’t be detected .(2)30 strains isolated from different areas in Hubei province were typed as HTNV amd SEOV。respectively.Those results suggested that:(1)The RT-heminested PCR was lea— sible for detectiong and typing HV in Hubei province.(2)Both HINV and SEOV were found in eoid— mic aro-.~.S of HFRS in Hubei province.Distinct hantaviruses could coexist in either different or the sam e geographic or ecological zones in Hubei province.

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