Primary Studies on DNA Imm unization of Hum an Rotavirus

PENG Shi—yong; WANG Jian-wei”; WEN Le-ying; HE Jin—sheng; HONG Tao

PDF
Cite
Share

facebook

twitter

google

linkedin

February 2002

PENG Shi—yong, WANG Jian-wei”, WEN Le-ying, HE Jin—sheng and HONG Tao. Primary Studies on DNA Imm unization of Hum an Rotavirus[J]. Virologica Sinica, 2002, 17(1): 38-41.

Citation: PENG Shi—yong, WANG Jian-wei”, WEN Le-ying, HE Jin—sheng, HONG Tao. Primary Studies on DNA Imm unization of Hum an Rotavirus .VIROLOGICA SINICA, 2002, 17(1) : 38-41.

人类轮状病毒基因DNA免疫的初步研究

1.
中国预防医学科学院扁毒学研究所．北京109052

摘要

：本文研究人类轮状病毒基因DNA免疫及应用。通过构建重组质粒pCI／x~7．pCl／x-p4及pCI／vp6，以肌注法导入BALB／c小鼠肌肉组织．其中pCI／vp7及pCl／x-p4能有救引起机体免疫应答，而且对轮状病毒wa株有一定中和效应。从车次试验的结果来看，人类轮状病毒DNA疫苗能作为预防病毒性4,JL腹泻的一种手段。
- 重组质粒
- , DNA免疫
- , 人类轮状病毒

Primary Studies on DNA Imm unization of Hum an Rotavirus

1.
1nstitute of Virology，China Academy of Preventive Medicine．Beijing 100052，China

Abstract

Abstract：The probability of DNA vaccine against human rotavirus was studied，by constructing three recombinant plasmid clones，pCI／vp7，pCI／vp4，and pCI／vp6，and transferring into TA muscle (quadriceps)of BALW C mous~．with intramuscular inoculation．The sera were collected and antibody activity was evaluated with E1 ISA and neutralizing test pCI／vp7，pCI／vp4，and pCI／vp6 have been successfully Constructed，moreover，pCl／vp7 has transfected 293 cells and the vp7 gene could expres thoroughly．Intramuscular inoculation of these plasmids induced efficient immuniation，and the anti— body induced by pCl／vp7 presents some definite protective of a strain of rotavirus Wa．As the results showed，DNA vaccine wil1 be a helpful means to protect children from severe acute diarrhea caused by Gro upA ro taviruse s．
- Recombinant plasmid
- , DNA vaccine
- , Human rotavirus

References
Proportional views

PDF

Article Metrics

Article views(3952) PDF downloads(836) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Primary Studies on DNA Imm unization of Hum an Rotavirus

1. 1nstitute of Virology，China Academy of Preventive Medicine．Beijing 100052，China

Abstract: Abstract：The probability of DNA vaccine against human rotavirus was studied，by constructing three recombinant plasmid clones，pCI／vp7，pCI／vp4，and pCI／vp6，and transferring into TA muscle (quadriceps)of BALW C mous~．with intramuscular inoculation．The sera were collected and antibody activity was evaluated with E1 ISA and neutralizing test pCI／vp7，pCI／vp4，and pCI／vp6 have been successfully Constructed，moreover，pCl／vp7 has transfected 293 cells and the vp7 gene could expres thoroughly．Intramuscular inoculation of these plasmids induced efficient immuniation，and the anti— body induced by pCl／vp7 presents some definite protective of a strain of rotavirus Wa．As the results showed，DNA vaccine wil1 be a helpful means to protect children from severe acute diarrhea caused by Gro upA ro taviruse s．