wu Juan。LI Jia—qi, SUN Mao-sheng, DAI Chang-bai and The Immune Response Induced by Recombinant Adenovirus with Administration of Interaperitonea Injection[J]. Virologica Sinica, 2002, 17(1): 51-55.
Citation: wu Juan。LI Jia—qi, SUN Mao-sheng, DAI Chang-bai, . The Immune Response Induced by Recombinant Adenovirus with Administration of Interaperitonea Injection .VIROLOGICA SINICA, 2002, 17(1) : 51-55.

腹腔注射重组腺病毒诱导的免疫反应

  • 摘要:为研究重组腺病毒接种实验动物后的免疫反应性,利用RT-PCR方法.从HAV的RNA中克隆了结构蛋白 基因插入穿梭质粒pXCXzNot I,通过磷酸钙一DNA共沉淀技术,将复制映陷型腺扁毒载体与线形化的pXCX2一CMVHAV 共转染293细胞。一系列检测方法证明产生了重组腺病毒rAdHAV。纯化后的rAdHAV滴度为1× 10 TCIDso/mL,腹腔注射免疫昆明种小白鼠后,可诱导产生抗HAV IgG和HAV中和抗体。复翻缺陷型腺病毒可 作为发展基因王程病毒疫苗载体的有效系统

The Immune Response Induced by Recombinant Adenovirus with Administration of Interaperitonea Injection

  • Abstract:In order to investigate the immunity of the recombinant adenoviruses inoculated to the ani— mals,structural gene from RNA of HAV was cloned by RT-PCR and inserted into the shuttle plasmid (pXCX2Not I).A replication-defective adenovirus was rescued in 293 packing cells via homologous re— combination of both ptasmid pXCX2·CMV—HAV and plMI7 containing AdS gertome with de[eforts of E1 region by calcium phosphate technique.A series of methods were employed to identify the generat— ed recombinant ademovirus(rAdI-IAV) The titer of rAdHAV stocks was up to I x 10 TCIDso/mL. The Kunming mice were administrated with rAdHAV by intraperitonea injeetion(i.P.).Anti—HAV IgG and HAV neutralizing antibodies were induced.In Conclusion,a recombinant replication—defective adenoviros is an efficient delivery system to develop recombinant virus vaccine.

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    The Immune Response Induced by Recombinant Adenovirus with Administration of Interaperitonea Injection

    • 1. Institute of Medical Biology Chinese Academy of Medical Sciences Peking Union Medical College,Kunming 650118,China

    Abstract: Abstract:In order to investigate the immunity of the recombinant adenoviruses inoculated to the ani— mals,structural gene from RNA of HAV was cloned by RT-PCR and inserted into the shuttle plasmid (pXCX2Not I).A replication-defective adenovirus was rescued in 293 packing cells via homologous re— combination of both ptasmid pXCX2·CMV—HAV and plMI7 containing AdS gertome with de[eforts of E1 region by calcium phosphate technique.A series of methods were employed to identify the generat— ed recombinant ademovirus(rAdI-IAV) The titer of rAdHAV stocks was up to I x 10 TCIDso/mL. The Kunming mice were administrated with rAdHAV by intraperitonea injeetion(i.P.).Anti—HAV IgG and HAV neutralizing antibodies were induced.In Conclusion,a recombinant replication—defective adenoviros is an efficient delivery system to develop recombinant virus vaccine.

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