Identification of the Receptor for PDV of Spodoptera Iitura M ultinucleoeapsid Nucleopolyhedrovirus

YIN Chong．YU Jian—xiu．PANG Yi*

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February 2002

YIN Chong．YU Jian—xiu．PANG Yi*, Identification of the Receptor for PDV of Spodoptera Iitura M ultinucleoeapsid Nucleopolyhedrovirus[J]. Virologica Sinica, 2002, 17(1): 66-68.

Citation: YIN Chong．YU Jian—xiu．PANG Yi*, . Identification of the Receptor for PDV of Spodoptera Iitura M ultinucleoeapsid Nucleopolyhedrovirus .VIROLOGICA SINICA, 2002, 17(1) : 66-68.

斜纹夜蛾多角体病毒包埋型病毒受体的鉴定

1.
中山大学生物防治国家重点实验室，广东广州510275

摘要

摘要：蔗糖密度梯虔离心法提纯斟纹夜蛾核多角体病毒(SpedopterⅡlit“m muhinudeocapsid nucl,mpolyhdrovinas． SphMNPV)包埋型病毒粒子(polyhedna-derived vir PDV)，以此病毒粒子作抗原．免疫家兔获得抗血清；用SDS裂解缓冲渣提取斜纹夜蛾幼虫中肠组织细胞总蛋白。采用病毒铺覆蛋白印迹技术(VOPBA)，利用抗PVD抗血清对病毒受体进行检测，结果表明斜纹夜蛾中肠细咆总蛋白中40kD、73kD、85kD的三种蛋白能够结台PDV。
- 斜纹夜蛾棱多角体病毒(SphMNPV)
- , 包埋型病毒粒子(PDV)
- , 受体蛋白
- , 病毒铺覆蛋白印违技术(VOP BA)

Identification of the Receptor for PDV of Spodoptera Iitura M ultinucleoeapsid Nucleopolyhedrovirus

1.
State Key Laboratory for Biocontrol，Zhongshaa University，Guangzhou 510275，China

Abstract

Abstract：This research provides the evidence about the identification of haculovirus receptors present in midgut cell lystes．Rabbit antiserum against purified PDV of SpltM NPV was produced Total midgut eel1 proteins were prepared with SDS lysis buffer with or wi出OUt 2-mere~,ptoethanal(2-ME)．After SDS—Polyacrylarnid gel eleetmphoresis(SDS—PAGE)，the midgut eel1 proteins were tranfered to nitro cellulose membrance．Transferred proteinS were renaturalized overnight with 4％ bovine serum albumin (BSA)．Then with PDV—specific antiserum．a virus overlay protein blot as~y(VOPBA)was performed to identify the protein that binds PDV of SpltMNPV．h was shown that PDV of SpltMNPV bound c0 three molecules of approximately 40kD，73kDand 85kD in midgut cel1 lysates．

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Identification of the Receptor for PDV of Spodoptera Iitura M ultinucleoeapsid Nucleopolyhedrovirus

1. State Key Laboratory for Biocontrol，Zhongshaa University，Guangzhou 510275，China

Abstract: Abstract：This research provides the evidence about the identification of haculovirus receptors present in midgut cell lystes．Rabbit antiserum against purified PDV of SpltM NPV was produced Total midgut eel1 proteins were prepared with SDS lysis buffer with or wi出OUt 2-mere~,ptoethanal(2-ME)．After SDS—Polyacrylarnid gel eleetmphoresis(SDS—PAGE)，the midgut eel1 proteins were tranfered to nitro cellulose membrance．Transferred proteinS were renaturalized overnight with 4％ bovine serum albumin (BSA)．Then with PDV—specific antiserum．a virus overlay protein blot as~y(VOPBA)was performed to identify the protein that binds PDV of SpltMNPV．h was shown that PDV of SpltMNPV bound c0 three molecules of approximately 40kD，73kDand 85kD in midgut cel1 lysates．