Cloning and Sequence Analysis of Buchnera GroEL Genefrom Endosybiotic Bacterium of M yzus persicae[J]. Virologica Sinica, 2002, 17(1): 69-72.
Citation: . Cloning and Sequence Analysis of Buchnera GroEL Genefrom Endosybiotic Bacterium of M yzus persicae .VIROLOGICA SINICA, 2002, 17(1) : 69-72.

桃蚜体内与病毒结合的共生菌Buchnera groEL基因的克隆和序列分析

  • 以桃蚜(Myzus persicae)杨凌生物型为材料,利用一对特 异性引物 ,用PCR的方法从桃蚜体内扩增出了内共生菌的Buchnera groEL基因,序 列 测定结果表明其长度为1 647bp,与GenBank中的桃蚜荷兰生物型、蜿豆蚜(Acyrthosiphon  pisum)日本生物型Buchnera GroEL基因长度相同,同源性分别为99%和91%;Buchnera GroEL-YL编码548个氨基酸,序列比较发现与Buchnera GroEL-NT仅有3个氨基酸的差异,即AA111MetLys、AA222ValMet and AA348GlnHis.

Cloning and Sequence Analysis of Buchnera GroEL Genefrom Endosybiotic Bacterium of M yzus persicae

  • Buchnera groEL gene of endosymbiotic bacterium (Buc hnera sp.) from Myzus persicae Yangling boitype (P.R.China) was isloated,a n d the complete nucleotides sequence was determined.It is indicated that Buchne ra groEL gene of M.persicae includes 1647 base pairs,has 99% and 91% sequ ce similarity with Buchnera groEL gene of M.persicae The Nether land bi otype (AF003957) and Acyrthosiphon pisum Japanese biotype (X61150),respectiv ely.Buchnera GroEL-YL contains 548 amino acide,and has 3 different amino ac ids comparing with Buchnera GroEL-NT,that is AA111MetLys、AA222ValMet and AA348GlnHis.

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    Cloning and Sequence Analysis of Buchnera GroEL Genefrom Endosybiotic Bacterium of M yzus persicae

    • 1. Department of Plant Protection, Nrothwest A&F University

    Abstract: Buchnera groEL gene of endosymbiotic bacterium (Buc hnera sp.) from Myzus persicae Yangling boitype (P.R.China) was isloated,a n d the complete nucleotides sequence was determined.It is indicated that Buchne ra groEL gene of M.persicae includes 1647 base pairs,has 99% and 91% sequ ce similarity with Buchnera groEL gene of M.persicae The Nether land bi otype (AF003957) and Acyrthosiphon pisum Japanese biotype (X61150),respectiv ely.Buchnera GroEL-YL contains 548 amino acide,and has 3 different amino ac ids comparing with Buchnera GroEL-NT,that is AA111MetLys、AA222ValMet and AA348GlnHis.

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