The Isolation and Identification of A New M ember of Tetraviridae Family from China

HU Yuan—yank; OUYANG Guo-jie; ZHANG ]ia-min; LI Li; wU Kai—lang YI Fu—ming; YIN Yi—nong

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February 2002

HU Yuan—yank, OUYANG Guo-jie, ZHANG ]ia-min, LI Li, wU Kai—lang YI Fu—ming and YIN Yi—nong. The Isolation and Identification of A New M ember of Tetraviridae Family from China[J]. Virologica Sinica, 2002, 17(1): 73-76.

Citation: HU Yuan—yank, OUYANG Guo-jie, ZHANG ]ia-min, LI Li, wU Kai—lang YI Fu—ming, YIN Yi—nong. The Isolation and Identification of A New M ember of Tetraviridae Family from China .VIROLOGICA SINICA, 2002, 17(1) : 73-76.

T_4病毒科一个新成员的分离鉴定

胡远扬欧阳国杰张珈敏李莉邬开朗易福明尹宜农 ,

1.
武汉大学病毒研究所湖北武汉

摘要

从松毛虫中分离一种球状二十面体病毒粒子。病毒直径为４４ｎｍ　。通过低温冷冻电镜技术和计算机图像处理技术，研究了该病毒衣壳的三维结构。结果表明　，病毒颗粒具双层结构，衣壳由２４０个亚基组成，分布于Ｔ＝４的二十面体上。病毒核酸地衣酚　反应呈阳性，联苯胺反应呈阴性结果。而紫外吸收实验则呈明显的单链特征，辅以酶消化实　验的结果，证实该病毒为单链ＲＮＡ病毒。琼脂糖电泳显示该病毒ＲＮＡ分子大小约为５．２ｋｂ。ＳＤＳ－聚丙烯酰胺电泳表明该病毒结构蛋白有一条大小约为５２ｋＤ主带和一条弱带（３９ｋＤ）。本病毒　是Ｔ－４病毒科的一名新成员，这是我国首次报告的Ｔ－４病毒科的病毒。
- T_4病毒
- , 化学特性
- , 分类
- , 松毛虫

The Isolation and Identification of A New M ember of Tetraviridae Family from China

1.
Institute of Virology，Wuahn University．Wuhan 430072，China

Abstract

A New member of Tetraviridae family was found and is olate d from Dendrolimus punetatus larvae in China.Purified virion is icosahedral spheroid with the diameter 44nm and 240 identical subunits in the particle with T=4 symmetry.The nucleic acid in the virion was determined to be single-strande d (ss) RNA by means of diphenylamine reaction,orcinol reaction and formaldehyde reaction.The viral RNA showed one band in agarose gel eletrophoresis (5.2kb).Usi n g SDS-PAGE,there were a major polypeptide band of molecular weight 52 kD and a small polypeptide at 39kD. According to the viral taxonomic principle,the virus belongs to Nudaurelia capensis β-like viruses in Tetraviridae.
- Tetravirus Chemical properties Viral taxon Dendrolimus punetatus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Isolation and Identification of A New M ember of Tetraviridae Family from China

1. Institute of Virology，Wuahn University．Wuhan 430072，China

Abstract: A New member of Tetraviridae family was found and is olate d from Dendrolimus punetatus larvae in China.Purified virion is icosahedral spheroid with the diameter 44nm and 240 identical subunits in the particle with T=4 symmetry.The nucleic acid in the virion was determined to be single-strande d (ss) RNA by means of diphenylamine reaction,orcinol reaction and formaldehyde reaction.The viral RNA showed one band in agarose gel eletrophoresis (5.2kb).Usi n g SDS-PAGE,there were a major polypeptide band of molecular weight 52 kD and a small polypeptide at 39kD. According to the viral taxonomic principle,the virus belongs to Nudaurelia capensis β-like viruses in Tetraviridae.