Comparison and Analysis 0f The CAV Fiber Protein Gene

FAN Quan; XIA Xian-zhu; QIU Wei; HUANG Geng; HE Hong; Yu Chun; QIAO Jun; wu Yin; ZHOU Xiao; YlN Zhen

PDF
Cite
Share

facebook

twitter

google

linkedin

February 2002

FAN Quan, XIA Xian-zhu, QIU Wei, HUANG Geng, HE Hong, Yu Chun, QIAO Jun, wu Yin, ZHOU Xiao and YlN Zhen. Comparison and Analysis 0f The CAV Fiber Protein Gene[J]. Virologica Sinica, 2002, 17(1): 82-86.

Citation: FAN Quan, XIA Xian-zhu, QIU Wei, HUANG Geng, HE Hong, Yu Chun, QIAO Jun, wu Yin, ZHOU Xiao, YlN Zhen. Comparison and Analysis 0f The CAV Fiber Protein Gene .VIROLOGICA SINICA, 2002, 17(1) : 82-86.

不同型犬腺病毒纤突蛋白基因序列的比较分析

1.
1成都军区联勤部军事医学研究所，昆明650032
2.
中国^民解放军农牧大学军事兽医研究所，长春130062
3.
东北农业大学，哈尔滨150030
4.
京军区军走队．北京昌平100021
5.
塔里术农垦大学，塔里木843300

摘要

摘要；根据已发表的CAV纤突基因序列，用PCR方法，对4个CAV 2毒株和4十CAV一1毒株的纤突基因进行了扩增和测序，测定的核甘酸序列经推导得到分别编码543和542个氨基酸 C’AV纤突蛋白垒序列。测定的CAV． 2比较表明：我国流行的CAV．2 SY强毒株与国外标准强毒株Tomnt0 A26／61株相同．其驯化致弱的毒株与驯化前相比在1134位发生碱基颇换。测定的CAV 1比较表明：我国流行的CAV-1株与标准强毒RI26l株差异相对较大，而国内cAv_l毒株互相之间相对差别较小 CAV．2与CAV，1纤突基因的同源性为80．48％。
- 犬腺病毒
- , 纤突基固
- , 纤突蛋白

Comparison and Analysis 0f The CAV Fiber Protein Gene

1.
1．TheM ilitaryMedicalInstitute ofCheng-DuMilitaryArea，Kunming 650032，China
2.
The VeterinaryInstitute．University of Agriculture and Animal Science．Cttangchun 130062 China
3.
The Northeastern Agriculture University，Harbin 150030．Ch i— Hd
4.
TheMilitary dogtroopofBeOingMlilitaryArea，BeljingChangping 100021，China
5.
TheTalimuAgriculture University ， Talimu 843300，China

Abstract

Abstract：Accordilag to CAV Fip genes sequence，4 paris of primer were designed tO amplify the Fip genes with PCR method．Complete Fip gene sequences of al1 CAV strains were shown tO be consisted of 1 629nt and 1 63lnt and encoded 542 and 543 amino acids from ATG start cod en．The deduced amino acid sequences of the Fip of the CAV一2 strains were compared one another and with standardard CAV strain extracted from the GenBank database．There are point mutation(transversion)occurring in at— tenuated SY—V6O and SY—V5 The Fip genes of CAV of virulent strain of different area were 8e— quenced There are less differences among domestic CAV一1 strains but more differences with foreign CAV．1 strains．Honology of Fip between CAV—l and CAV一2 iS 80．48％．
- Canine adenovirus
- , Fiber protein gene

References
Proportional views

PDF

Article Metrics

Article views(4605) PDF downloads(875) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparison and Analysis 0f The CAV Fiber Protein Gene

1. 1．TheM ilitaryMedicalInstitute ofCheng-DuMilitaryArea，Kunming 650032，China
2. The VeterinaryInstitute．University of Agriculture and Animal Science．Cttangchun 130062 China
3. The Northeastern Agriculture University，Harbin 150030．Ch i— Hd
4. TheMilitary dogtroopofBeOingMlilitaryArea，BeljingChangping 100021，China
5. TheTalimuAgriculture University ， Talimu 843300，China

Abstract: Abstract：Accordilag to CAV Fip genes sequence，4 paris of primer were designed tO amplify the Fip genes with PCR method．Complete Fip gene sequences of al1 CAV strains were shown tO be consisted of 1 629nt and 1 63lnt and encoded 542 and 543 amino acids from ATG start cod en．The deduced amino acid sequences of the Fip of the CAV一2 strains were compared one another and with standardard CAV strain extracted from the GenBank database．There are point mutation(transversion)occurring in at— tenuated SY—V6O and SY—V5 The Fip genes of CAV of virulent strain of different area were 8e— quenced There are less differences among domestic CAV一1 strains but more differences with foreign CAV．1 strains．Honology of Fip between CAV—l and CAV一2 iS 80．48％．