The Studies on Expression of 1 197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product
Abstract: In previous report we have cloned a putative early and late transcriptional gene 1 197 of prawn baculovirus.In order to detect the biological function of this gene ,1197 gene was inserted into expressing vector pGEX-3X.The fusion protein expressed with high yield gua$obtained,but it~1)as foundthattheAsion proteinlocatesininclusion bodies ofE.coli andit causod troubleinitspurfica— tion.Wefoundthatthe expressed productwithM .of 66kD could bepurifiedfrom inclusion hod— ies by using a serious of treatments of inclusion bodies r~,ith ug’ea and guanidine hydrochloride and then by sequential FPLC chromatography.