YING Zhi-wei, ZHOU Guo-ying.GONG Zu-Xun and The Studies on Expression of 1 197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product[J]. Virologica Sinica, 2002, 17(1): 92-95.
Citation: YING Zhi-wei, ZHOU Guo-ying.GONG Zu-Xun, . The Studies on Expression of 1 197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product .VIROLOGICA SINICA, 2002, 17(1) : 92-95.

对虾白斑病毒1197基因的表达和产物纯化的研究

  • 1993年以来,我国沿海省份相继发生养殖对虾 大批死亡现象,损失严重,引起国内外研究者的重 视。经过一段时间的努力研究后,我们报导了引起 该病的病原是一种无包含体的杆形病毒⋯ 。此后, 该病毒的一些其他特性及引起对虾的病理学及组织 学的变化又陆续见诸于报导_2, 。前文中我们报导 了该病毒的一个早晚期基因1197的克隆和序列分 析,并针对此基因设计了二个核酶,在体外成功地对 此基因进行了切割_4 J。本文报导我们对该基因进 行了原棱表达,并对表达产物进行了纯化的研究, 期为阐明该基因表达产物的功能打下进一步研究的 基础。

The Studies on Expression of 1 197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product

  • In previous report we have cloned a putative early and late transcriptional gene 1 197 of prawn baculovirus.In order to detect the biological function of this gene ,1197 gene was inserted into expressing vector pGEX-3X.The fusion protein expressed with high yield gua$obtained,but it~1)as foundthattheAsion proteinlocatesininclusion bodies ofE.coli andit causod troubleinitspurfica— tion.Wefoundthatthe expressed productwithM .of 66kD could bepurifiedfrom inclusion hod— ies by using a serious of treatments of inclusion bodies r~,ith ug’ea and guanidine hydrochloride and then by sequential FPLC chromatography.

  • 加载中
  • 加载中

Article Metrics

Article views(3708) PDF downloads(861) Cited by(0)

Related
Proportional views
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    The Studies on Expression of 1 197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product

    • 1. The Institute of Biochemistry and cell Biology,Chinese Academy Sciences,Shanghai 200031,China

    Abstract: In previous report we have cloned a putative early and late transcriptional gene 1 197 of prawn baculovirus.In order to detect the biological function of this gene ,1197 gene was inserted into expressing vector pGEX-3X.The fusion protein expressed with high yield gua$obtained,but it~1)as foundthattheAsion proteinlocatesininclusion bodies ofE.coli andit causod troubleinitspurfica— tion.Wefoundthatthe expressed productwithM .of 66kD could bepurifiedfrom inclusion hod— ies by using a serious of treatments of inclusion bodies r~,ith ug’ea and guanidine hydrochloride and then by sequential FPLC chromatography.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return