Cloning and Analyzing a New PEBP Like Protein Gene in Human Fibroblast Cell Infected by HSV I

W ANG Jlong．LIU Long—Ding; SUN M ing; DONG Cheng-Hong; WANG Li—Chun; WANG Jing-Jing．LI Qi—han

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April 2002

W ANG Jlong．LIU Long—Ding, SUN M ing, DONG Cheng-Hong, WANG Li—Chun, WANG Jing-Jing．LI Qi—han, Cloning and Analyzing a New PEBP Like Protein Gene in Human Fibroblast Cell Infected by HSV I[J]. Virologica Sinica, 2002, 17(2): 97-101.

Citation: W ANG Jlong．LIU Long—Ding, SUN M ing, DONG Cheng-Hong, WANG Li—Chun, WANG Jing-Jing．LI Qi—han, . Cloning and Analyzing a New PEBP Like Protein Gene in Human Fibroblast Cell Infected by HSV I .VIROLOGICA SINICA, 2002, 17(2) : 97-101.

在HSV I感染细胞中类PEBP蛋白基因的克隆及分析

1.
中国医学科学院．中国协和医科大学。医学生物学研究所。云南昆明650118

摘要

摘要：在细胞对病毒感染后的基因反应研究中，从克隆的HSVI感染后细胞特异cDNA文库中筛选出一个705bp 克隆基因一HsP 714，基因测序分析表明为一新的全基因序列(Gene Bank Accession Number：AF372624)，含有完整的ORF框架，编码126个氨基酸；信息生物学分析结果表明该蛋白含有磷脂酰乙醇胺结合蛋白(PEBP)结梅域，与植物、哺乳动物等多物种类CEN家族产物有高度的同源性．此外该基因的进化分析结果表明此基因更接近于植物的类CEN家族。据此推测，该基因可能是人PEBP基因家族中具有特殊意义的一个新成员。
- 类磷脂酰乙醇胺结合蛋白
- , HSVI病毒感染
- , 克隆

Cloning and Analyzing a New PEBP Like Protein Gene in Human Fibroblast Cell Infected by HSV I

1.
Department of Viral and Immunology，Institute of medical Bio1~gy，CAMS and PUMC，Kunmlng 650118，China

Abstract

In our study on the gene response to virus infection，a colony of 705bp—HSP一714 was se— lected from the cell specific cDNA library induced by HSVI infection．The gene sequence analysis re· vealed that it was a new complete gene sequence(Gene Bank Accession number~AF372624)with a complete ORF encoding 126 amino acids．The biological informatics aiualysis demonstrated that this pmtein contained a phosphatidylethanolamine—binding protein(PEBP)like domain，which had a high homology to the CEN—like family of plant and mam malian ete．genome．Furthermore，the evolution tree demonstrates that this gene shows more homologous to CEN—like family Of plant Therefore．it can be predicted that this gene might be a new member of human PEBP—like family with special significance
- PEBP like pmtein：HSVI infection
- , Gene cloning

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Analyzing a New PEBP Like Protein Gene in Human Fibroblast Cell Infected by HSV I

1. Department of Viral and Immunology，Institute of medical Bio1~gy，CAMS and PUMC，Kunmlng 650118，China

Abstract: In our study on the gene response to virus infection，a colony of 705bp—HSP一714 was se— lected from the cell specific cDNA library induced by HSVI infection．The gene sequence analysis re· vealed that it was a new complete gene sequence(Gene Bank Accession number~AF372624)with a complete ORF encoding 126 amino acids．The biological informatics aiualysis demonstrated that this pmtein contained a phosphatidylethanolamine—binding protein(PEBP)like domain，which had a high homology to the CEN—like family of plant and mam malian ete．genome．Furthermore，the evolution tree demonstrates that this gene shows more homologous to CEN—like family Of plant Therefore．it can be predicted that this gene might be a new member of human PEBP—like family with special significance