The Analysis of HEV Genotypes Isolated from Sporadic Acute Hepatitis E in Shanghai

LAN Hai; W ANG; u chun; ZHANG Hua; PENG Hui; LIN Jing; GU Wen; WU Xing; LI He

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April 2002

LAN Hai, W ANG, u chun, ZHANG Hua, PENG Hui, LIN Jing, GU Wen, WU Xing and LI He. The Analysis of HEV Genotypes Isolated from Sporadic Acute Hepatitis E in Shanghai[J]. Virologica Sinica, 2002, 17(2): 106-109.

Citation: LAN Hai, W ANG, u chun, ZHANG Hua, PENG Hui, LIN Jing, GU Wen, WU Xing, LI He. The Analysis of HEV Genotypes Isolated from Sporadic Acute Hepatitis E in Shanghai .VIROLOGICA SINICA, 2002, 17(2) : 106-109.

上海部分地区戊型肝炎病毒(HEV)基因型的分析

1.
1.中国药品生物制品柱定所疫苗二室．北京LO0050
2.
上海市传染病医院．上海200083

摘要

为了解戊型肝炎病毒(HEY)在上海部分地区流行的基因型+采用RT nPCR的方法检验35倒急性散发性戊型肝炎患者中HEV RNA，并对阳性产物进行克隆测序，然后对其基因型进行分析。结果显示在35例急性散发性皮型肝炎患者中PCR阳性为9倒，测序证实8倒为HEV的基因序列；其中l倒为HEVl型，7倒为HEV 4型。提示在上海部分地区的息性散发性戊型肝炎中以HEV 4型感染为主
- 戊型肝炎病毒
- , 基因型
- , 聚合酵链式反应

The Analysis of HEV Genotypes Isolated from Sporadic Acute Hepatitis E in Shanghai

1.
1．Department ofHepatitis，HationaEInstitutefo，theControl ofPharmaceutical andBiologicalProducts，Beljing 100050．China 2． infectious Hospital，Shanghai 200083，China

Abstract

In order to analyze HEV genotypes isolated from sporadic acute hepatitis E in Shanghai， HEV RNAs were detected with RT—nPCR and the positive PCR products were cloned and sequenced The genotypes of the isolated sequences were analyzed with the molecular biological software．The re— suits showed that 9 of 35 sera from patients diagnosed as acute sporadic hepatitis E were positive for RT-nPCR．8 of 9 PCR positive products were confirmed to be related to HEV sequence by sequencing． 1 of 8 sequences belongs to HEV genotype l and 7 of 8 sequences belongs to HEV genotype 4 This in— dicates that the infection rate of HEV genotype 4 is higher than that of HEV genotype 1 among the pa— tients with the diagnosis of acute spo radic hepatitis in Shanghai．
- Hepatitis E virus
- , Genotype
- , Polymerase chain reaction

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Analysis of HEV Genotypes Isolated from Sporadic Acute Hepatitis E in Shanghai

1. 1．Department ofHepatitis，HationaEInstitutefo，theControl ofPharmaceutical andBiologicalProducts，Beljing 100050．China 2． infectious Hospital，Shanghai 200083，China

Abstract: In order to analyze HEV genotypes isolated from sporadic acute hepatitis E in Shanghai， HEV RNAs were detected with RT—nPCR and the positive PCR products were cloned and sequenced The genotypes of the isolated sequences were analyzed with the molecular biological software．The re— suits showed that 9 of 35 sera from patients diagnosed as acute sporadic hepatitis E were positive for RT-nPCR．8 of 9 PCR positive products were confirmed to be related to HEV sequence by sequencing． 1 of 8 sequences belongs to HEV genotype l and 7 of 8 sequences belongs to HEV genotype 4 This in— dicates that the infection rate of HEV genotype 4 is higher than that of HEV genotype 1 among the pa— tients with the diagnosis of acute spo radic hepatitis in Shanghai．