Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes

LI Zhi; YANG Pin; LIU Hui; LI Wen—xin

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April 2002

LI Zhi, YANG Pin, LIU Hui and LI Wen—xin. Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes[J]. Virologica Sinica, 2002, 17(2): 114-118.

Citation: LI Zhi, YANG Pin, LIU Hui, LI Wen—xin. Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes .VIROLOGICA SINICA, 2002, 17(2) : 114-118.

复制缺陷型人泡沫逆转录病毒载体构建和外源基因表达

1.
武汉大学生命科学学院，湖北武汉430072

摘要

在人泡沫逆转录病毒(human foamy virus．HFv) 病毒全长克隆pHRSV13的基础上，缺失病毒基因组的en 基因和6 基因，构建了一个表达标记基因。。和报道基因gYP的重组人泡沫病毒载体质粒pGPSNI．GFP。在与辅助质粒pZXGp共转染情况下．得到复制映陷型的重组病毒颗粒GPSNI．GFP。该病毒颗粒可以感染多种宿主细胞，将携带的外源基固整合于细胞染色体DNA上并实现表达。
- 逆转录病毒
- , 人泡沫病毒
- , 载体

Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes

1.
College of life sciences．Wuhan University．Wuhan 430072．China

Abstract

A replication—defective vector(pGPSN1一GFP)base on an infections molecular clone of HFV (pHSRV13)was constructed by deletion and replacement of the en’u and bet genes with exprssion cas— setts for neomycin—resistance and EGFP．Vector stocks weir,prodaced by cationic lipid reagents co — transfection of BHK一21 cells with pGPSNI—GFP and helper plasmid P△ GP These vectors are able to tra~sfer foreign genes into several vertebrate cells and steadily express them ．
- Retrovirus
- , Human foamy virus
- , Vector

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Constraction of Replication—defective Hum an Foamy Virus Vector Directing Expression of Foreign Genes

1. College of life sciences．Wuhan University．Wuhan 430072．China

Abstract: A replication—defective vector(pGPSN1一GFP)base on an infections molecular clone of HFV (pHSRV13)was constructed by deletion and replacement of the en’u and bet genes with exprssion cas— setts for neomycin—resistance and EGFP．Vector stocks weir,prodaced by cationic lipid reagents co — transfection of BHK一21 cells with pGPSNI—GFP and helper plasmid P△ GP These vectors are able to tra~sfer foreign genes into several vertebrate cells and steadily express them ．