Genetic Organization of the EcoR I-G Region of the Spodoptera litura Nucleopolyhedrovirus

LI Zhen; LONG Qing-xing; YUAN Mei-jing; HE Xiong—lei; PANG Yi; WANG Xun—zhang

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April 2002

LI Zhen, LONG Qing-xing, YUAN Mei-jing, HE Xiong—lei, PANG Yi and WANG Xun—zhang. Genetic Organization of the EcoR I-G Region of the Spodoptera litura Nucleopolyhedrovirus[J]. Virologica Sinica, 2002, 17(2): 119-124.

Citation: LI Zhen, LONG Qing-xing, YUAN Mei-jing, HE Xiong—lei, PANG Yi, WANG Xun—zhang. Genetic Organization of the EcoR I-G Region of the Spodoptera litura Nucleopolyhedrovirus .VIROLOGICA SINICA, 2002, 17(2) : 119-124.

斜纹夜蛾核多角体病毒(SpltNPV)基因组EcoR I-G片段的序列分析

1.
中山大学生物防治国家重点实验室．广东广州510275

摘要

摘要：斜纹夜蛾棱多角体病毒(SP1rNPV)基因组EcoR I-G片段垒长7 450hp，包括7个开放读码框：vp39、 1ef-4、 cg30、p91、v933、tlp20、AcMNPV ORF81同源基因和一个同源区(hr) 基因组排列比较分析发现，这些基因在基因组中的排列，在所有已知序列的杆状病毒中都比较保守。
- 斜纹夜蛾核多角体病毒
- , vp39
- , lef-4同源区
- , 序列分析

Genetic Organization of the EcoR I-G Region of the Spodoptera litura Nucleopolyhedrovirus

1.
State Key Laboratory or Biocomrot，Zhongshan Unive~ity，Guangzhou 510275．China

Abstract

In order to investigate the genomic organzation of the S／,o’ptera litura multinucleoeapsid nucleopolyhedrovirus(SphNPV)，the EcoR 1-G[ragment located at map units(mu)49 2-54．1 ol the viral genome was sequenced．The fragment contains 2 partial and 5 complete open reading frames (ORF)representing the viral capsid protein gene p 39 and P 91，[ate expression factor 4(z 4)， cg30 gene，tlp20 gene，including homologous region(hr)+Sequence analysis further revealed that these ORFs have the same conserved motifs and gene structure as those observed in 【heir homologues from other baculovirus do．The genomic arrangement of the ORFs in the SpltNPV is similar in aI1 the bac— oloviirus genome，it maybe reveals that this fragment is the conserved part in the baculovirus

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Genetic Organization of the EcoR I-G Region of the Spodoptera litura Nucleopolyhedrovirus

1. State Key Laboratory or Biocomrot，Zhongshan Unive~ity，Guangzhou 510275．China

Abstract: In order to investigate the genomic organzation of the S／,o’ptera litura multinucleoeapsid nucleopolyhedrovirus(SphNPV)，the EcoR 1-G[ragment located at map units(mu)49 2-54．1 ol the viral genome was sequenced．The fragment contains 2 partial and 5 complete open reading frames (ORF)representing the viral capsid protein gene p 39 and P 91，[ate expression factor 4(z 4)， cg30 gene，tlp20 gene，including homologous region(hr)+Sequence analysis further revealed that these ORFs have the same conserved motifs and gene structure as those observed in 【heir homologues from other baculovirus do．The genomic arrangement of the ORFs in the SpltNPV is similar in aI1 the bac— oloviirus genome，it maybe reveals that this fragment is the conserved part in the baculovirus