Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera

FENG Zong—di; CHEN Xin—wen; Just M ．Vlak; HU Zhi—hong

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April 2002

FENG Zong—di, CHEN Xin—wen, Just M ．Vlak and HU Zhi—hong. Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera[J]. Virologica Sinica, 2002, 17(2): 125-131.

Citation: FENG Zong—di, CHEN Xin—wen, Just M ．Vlak, HU Zhi—hong. Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera .VIROLOGICA SINICA, 2002, 17(2) : 125-131.

中国棉铃虫核多角体病毒基因组Xba I-I片段的序列分析

1.
1．中国科学院武汉病毒研究所无脊椎动物病毒学联台实验室，湖北武汉430071
2.
Department ofVirology，Wageningen Universilty．Binnen haven l1．6709 PD W ageningen，the Neth~rhnds

摘要

报道丁棉铃虫单核衣壳核多角体病毒(HaSNPV)XbaI-I片段的序列结构。该片段在基因组上定位于L8 4— 22-8 rn u，包括8十完整的开放阅读框架和p47的5 端部分序列：其中“biquitin 39K／pp31， r一11， 47， Ac34，Ac38和Lse125 honrologue 7十基因是杆状病毒的同源基因，另外两十0rJ507和0r厂1 080是HaSNPV的特有基因，且具有典型的早期表达基因启动于的特征．经软件分析发现这两个基因推导的产物具有典型的跨膜压结掏。序列比较分析表明， biq“iti 基因与其它杆状病毒的同源基凼有相同的保守区， 39K／pp31具有和其它杆状病毒回源基因不同的启动子结构
- 棉钟虫核多角体病毒
- , 39K／pp31
- , led一11
- , p47
- , biquitin

Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera

1.
The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.

Abstract

The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.
- Helicoverl~ armigera single nucleocapsid nucteopolyhedrovirus
- , Hbiquitin
- , 39K／pp31： tel-11
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nllcle0polyhedr0Virus of Helicoverpa armigera

1. The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.

Abstract: The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.