Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle

WANG Ying; YU Ze; YAO Han; TAO De; CHEN Qu

PDF
Cite
Share

facebook

twitter

google

linkedin

April 2002

WANG Ying, YU Ze, YAO Han, TAO De and CHEN Qu. Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle[J]. Virologica Sinica, 2002, 17(2): 132-136.

Citation: WANG Ying, YU Ze, YAO Han, TAO De, CHEN Qu. Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle .VIROLOGICA SINICA, 2002, 17(2) : 132-136.

苜蓿银纹夜蛾核型多角体病毒的感染对Sf9细胞周期的影响

1.
1华中师范大学昆虫研究所．湖北武汉430079：2华中科技大学同济附匾医院分子实验中心．朝北武汉430030

摘要

摘要：病毒的感染导致细胞内部发生一系列变化。应用流式细胞仪FACS的荧光检测，测出Sf9细胞完成整个周期循环大约需要I8h．G1、S、GfM 各时相的时间间隔约为6h；AcNPV 感染Sf9细胞1218h，细咆被抑制于GfM 期；SD细胞同步于G一／S期后释放细咆并用AcNPV感染，12h后．2／3的细咆处于GfM期．1／3的细胞处于S期。
- 苜蓿银纹夜蛾核型多角体病毒
- , 细胞周期
- , 流式细胞仪分析
- , 周期抑制

Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle

1.
Institue of Entomology．Central China Normal University，Wuhan 430079，China

Abstract

Baculovirus infection resulted in many changes in host cells.The progress of the cell cycle of Spodoptera frgiperda IPLB-Sf21-AE clonal isolate 9 (Sf9) cells was directed by Flow cytometry analysis(FACS).The results showed that the whole time of the cell cycle was about 18 hours and the gapes between every two phases were about 6 hours.At 12-18 hours post AcNPV infection,Sf9 cells were arrested in G\-2/M phase.When the cells in G\-1/S phase synchronized by druge were infected by AcNPV,2/3 of the cells was in G\-2/M phase and 1/3 of the cells in S phase.
- AcNPV
- , Cell cycle
- , FAGS
- , Cell cycle arresting

References
Proportional views

PDF

Article Metrics

Article views(3986) PDF downloads(919) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Affection of Infection of Autographa californica nucleopolyhedroviruson Sf9 Cell Cycle

1. Institue of Entomology．Central China Normal University，Wuhan 430079，China

Abstract: Baculovirus infection resulted in many changes in host cells.The progress of the cell cycle of Spodoptera frgiperda IPLB-Sf21-AE clonal isolate 9 (Sf9) cells was directed by Flow cytometry analysis(FACS).The results showed that the whole time of the cell cycle was about 18 hours and the gapes between every two phases were about 6 hours.At 12-18 hours post AcNPV infection,Sf9 cells were arrested in G\-2/M phase.When the cells in G\-1/S phase synchronized by druge were infected by AcNPV,2/3 of the cells was in G\-2/M phase and 1/3 of the cells in S phase.