cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu

WANG Zhanghui; ZHOU Yi-jun; FAN Yong jian．CHENG Zhao-bang; ZHANG Wen—hui

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April 2002

WANG Zhanghui, ZHOU Yi-jun, FAN Yong jian．CHENG Zhao-bang, ZHANG Wen—hui and cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu[J]. Virologica Sinica, 2002, 17(2): 142-144.

Citation: WANG Zhanghui, ZHOU Yi-jun, FAN Yong jian．CHENG Zhao-bang, ZHANG Wen—hui, . cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu .VIROLOGICA SINICA, 2002, 17(2) : 142-144.

江苏水稻黑条矮缩病毒S10的cDNA克隆序列分析

1.
江苏省农业科学院植物保护研究所，江苏南京,210014

摘要

从来自江苏连云港并在本实验室保存的水稻黑条矮缩病毒接种的病株玉米中提取dsRNA．采用改进的单引物扩增技术获得丁病毒基因组片段$10的cDNA克隆井测定了其垒序列。结果表明$10全长l 80lbp．含有一个 ORF．组织结构与日本报道的RBSDV基本一致，核苷酸和推导的氮基酸序列与MRDV的相似性分别为87 5％和 92 6％．与RBSDV 的相似性分别为93 3％和96 4％。该研究也为病毒dsRNA克隆和序列分析奠定丁基础
- 水稻黑条矮缩病毒
- , cDNA克隆
- , SIO
- , 序列分析

cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu

1.
Jiangsu Academy Agricultural Sciences,Nanjing 210014．China

Abstract

An isolate of rice black-streaked dwarf Fijivirus originally from Lianyuangng，Jiangsu，China was used tO inoculated maize tO propagate the virus Improved single primer amplification technique was adopted tO clone the vira1 genomic dsRNA SIO for sequence determination The result showed：the full length of S10 is 1 801 bp and has the same organization with M RDV in Italy and RBSDV in Japan The similarity of nucleotide and predicted protein weDe 87 5％ and 92．6％ with MRDV． 93．3％ and 96．4％ with RBSDV ．This study provides a new methodforthe sequence determination of viral dsRNA
- Rice black—streaked dwarf Fijivirus
- , cDNA clone
- , Sl0
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu

1. Jiangsu Academy Agricultural Sciences,Nanjing 210014．China

Abstract: An isolate of rice black-streaked dwarf Fijivirus originally from Lianyuangng，Jiangsu，China was used tO inoculated maize tO propagate the virus Improved single primer amplification technique was adopted tO clone the vira1 genomic dsRNA SIO for sequence determination The result showed：the full length of S10 is 1 801 bp and has the same organization with M RDV in Italy and RBSDV in Japan The similarity of nucleotide and predicted protein weDe 87 5％ and 92．6％ with MRDV． 93．3％ and 96．4％ with RBSDV ．This study provides a new methodforthe sequence determination of viral dsRNA