Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain

JIANG Yan ．HOU Yu—feng; CHEN De—sheng; CHEN Pu yan “

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April 2002

JIANG Yan ．HOU Yu—feng, CHEN De—sheng, CHEN Pu yan “ and Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain[J]. Virologica Sinica, 2002, 17(2): 149-152.

Citation: JIANG Yan ．HOU Yu—feng, CHEN De—sheng, CHEN Pu yan “, . Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain .VIROLOGICA SINICA, 2002, 17(2) : 149-152.

伪狂犬病病毒上海株gE和gI基因的克隆及缺失转移载体的构建

1.
1南京农业大学动物医学院．江苏南京210095
2.
南京出入境检验检疫局．江苏南京210001

摘要

根据已发表的伪狂犬病病毒(Pseudorabies Virus．PRV)I’Igt和gE基因序列．设计两对引物用PCR方法扩增出PRV—SH 和gE基因，将其克隆 pUC18载体中．获得了缺失部分基因gI的转移载体质粒，命名为pgEI
- 伪狂犬病病毒
- , gl基因
- , gE基因

Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain

1.
1．College of Veterinary Medicine，Nanjing Ag iculture University，Nanjing 210095，China
2.
Nanjin Entry-Entry lnspection and quranting Beaury Nanjing 210001 China

Abstract

According to the Genbank open sequence，two pairs of primer were designed in order to am — plify gE gene and partial gI gene of pseudorabies virus Shanghai strain，respectively．The g￡gene and partial gI gene were obtained by po]ymerase chain reaction(PCR)，subsequently cloned into pGEM T vector．Then both of them were inserted into pUCI8 vector．The recombinant plasmid pgEI deleted part of gI gene was constructed．
- Pseudorabies virus
- , gl gene
- , gene

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain

1. 1．College of Veterinary Medicine，Nanjing Ag iculture University，Nanjing 210095，China
2. Nanjin Entry-Entry lnspection and quranting Beaury Nanjing 210001 China

Abstract: According to the Genbank open sequence，two pairs of primer were designed in order to am — plify gE gene and partial gI gene of pseudorabies virus Shanghai strain，respectively．The g￡gene and partial gI gene were obtained by po]ymerase chain reaction(PCR)，subsequently cloned into pGEM T vector．Then both of them were inserted into pUCI8 vector．The recombinant plasmid pgEI deleted part of gI gene was constructed．