Sequence Comparison and Analysis of L Gene from A Foot—and·month Disease Virus Strain China／99

ZHANG Xian; ZHAO Qi; IAU Zai; LIU Xiang; CHANG Hui; JIANG Peng; fei CHENG; LI Dong; WEI Huai; XIE Qing

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April 2002

ZHANG Xian, ZHAO Qi, IAU Zai, LIU Xiang, CHANG Hui, JIANG Peng, fei CHENG, LI Dong, WEI Huai and XIE Qing. Sequence Comparison and Analysis of L Gene from A Foot—and·month Disease Virus Strain China／99[J]. Virologica Sinica, 2002, 17(2): 158-162.

Citation: ZHANG Xian, ZHAO Qi, IAU Zai, LIU Xiang, CHANG Hui, JIANG Peng, fei CHENG, LI Dong, WEI Huai, XIE Qing. Sequence Comparison and Analysis of L Gene from A Foot—and·month Disease Virus Strain China／99 .VIROLOGICA SINICA, 2002, 17(2) : 158-162.

口蹄疫病毒株China／99 L区段核苷酸序列测定及比较分析

1.
中国农业科学院兰州兽医研究所．兰州730046

摘要

以口蹄疫病毒株China/99 RNA为模板．反转录并扩增目的cDNA．然后与pGEM．T Easy载捧连接并转化 JM109菌株．提取的重组质粒用电泳、PCR amp~~和EwR1酶切法鉴定。该毒株与A10、O K、O1C amp。s和TW45 毒株的桉苷酸序列差异率分别为15 27％、15．56％、15 56％和15 49％氡基酸序列差异率分别为8 29％、8.76％、9．22％和l0．14％。五十毒株的L／P1连接处均为苷氨酸(Gly}／异亮氡酸(Ue)。序列比较表明．T—C、AG 和AC转换率较高．是点突变的热点核苷酸．是影响氨基酸稳定的因素之一第43 53、95105、108．111、 146153、 161173、183·188和182187区域扳有可能是L蛋白酶的活性中心．第48位的H、5l的c、65位的E、95位的H、109 位的H、138位的H、148位的H和165位的E可能是L蛋白酶的活性位点．它们在维持蛋白质的空间掏像和功能方面具有重要作用。
- 口蹄疫病毒
- , L基因
- , 序列比较分析

Sequence Comparison and Analysis of L Gene from A Foot—and·month Disease Virus Strain China／99

1.
Lanzhou Veterinary Research Institute．CAAS．Lanzhou 730046．China

Abstract

Foot and mouth disease virus strain China／99 RNA5 were used as templet for RT—PCR．The amplifled eDNA products were cloned into pGEM—T Easy Vectors and transformed into JM109．The re— combinant plasroids were identified by electrophoresis．E∞R l cleavage，and PCR The nucleotide and amino acid sequences were compared with the I，genes of the other four reference strains．The resuIts shown that the differential ratio of L gene of China／99 with A10．OIK，OlCaropos and TW45 strains was l5 27％，15．56％．15 56％ and 15 49％ respectively；and that of amio acid se0uences was 8．29％．8．76％。9 22％ and 10 14％ respectively．All the conjunctions of L／el for five fot—and— mouth viruses are Gly／lie．The higher conversions in T．C．A-G and A-c mostly affect the stabgle of amino acid residuses． The regions of 43．53th． 95．105th， 108—11lth， 146—153th 。161—173th． 183— 188th and 182·187[h in I protease roost probably are the active sites that play an important role 1n keeping configuration andfunction of proteins，andH 8．C5l，E65，H95．H109，Hl38，Hl48 andEI65may be active amino acids
- FMDV
- , L gene：Sequence analyzing

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequence Comparison and Analysis of L Gene from A Foot—and·month Disease Virus Strain China／99

1. Lanzhou Veterinary Research Institute．CAAS．Lanzhou 730046．China

Abstract: Foot and mouth disease virus strain China／99 RNA5 were used as templet for RT—PCR．The amplifled eDNA products were cloned into pGEM—T Easy Vectors and transformed into JM109．The re— combinant plasroids were identified by electrophoresis．E∞R l cleavage，and PCR The nucleotide and amino acid sequences were compared with the I，genes of the other four reference strains．The resuIts shown that the differential ratio of L gene of China／99 with A10．OIK，OlCaropos and TW45 strains was l5 27％，15．56％．15 56％ and 15 49％ respectively；and that of amio acid se0uences was 8．29％．8．76％。9 22％ and 10 14％ respectively．All the conjunctions of L／el for five fot—and— mouth viruses are Gly／lie．The higher conversions in T．C．A-G and A-c mostly affect the stabgle of amino acid residuses． The regions of 43．53th． 95．105th， 108—11lth， 146—153th 。161—173th． 183— 188th and 182·187[h in I protease roost probably are the active sites that play an important role 1n keeping configuration andfunction of proteins，andH 8．C5l，E65，H95．H109，Hl38，Hl48 andEI65may be active amino acids